BMRB Entry 6428

Title:
Probing the pH-dependent structural features of a-KTx12.1, a potassium channel blocker from the scorpion Tityus serrulatus
Deposition date:
2004-12-10
Original release date:
2006-08-11
Authors:
Oyama, Sergio; Pristovsek, Primoz; Franzoni, Lorella; Pertinhez, Thelma; Schinina, Eugenia; Luecke, Christian; Rueterjans, Heinz; Arantes, Eliane; Spisni, Alberto
Citation:

Citation: Oyama, Sergio; Pristovsek, Primoz; Franzoni, Lorella; Pertinhez, Thelma; Schinina, Eugenia; Luecke, Christian; Rueterjans, Heinz; Arantes, Eliane; Spisni, Alberto. "Probing the pH-dependent structural features of alpha-KTx12.1 a potassium channel blocker from the scorpion Tityus serrulatus"  Protein Sci. 14, 1025-1038 (2005).
PubMed: 15772309

Assembly members:

Assembly members:
alpha-KTx12.1, polymer, 40 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Brazilian scorpion   Taxonomy ID: 6887   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Tityus serrulatus

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
alpha-KTx12.1: WCSTCLDLACGASRECYDPC FKAFGRAHGKCMNNKCRCYT

Data sets:
Data typeCount
1H chemical shifts700

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-KTx12.11

Entities:

Entity 1, alpha-KTx12.1 40 residues - Formula weight is not available

1   TRPCYSSERTHRCYSLEUASPLEUALACYS
2   GLYALASERARGGLUCYSTYRASPPROCYS
3   PHELYSALAPHEGLYARGALAHISGLYLYS
4   CYSMETASNASNLYSCYSARGCYSTYRTHR

Samples:

sample_1: alpha-KTx12.10.5 – 0.7 mM

condition_1: pH: 4.5; temperature: 308 K

condition_2: pH: 6.0; temperature: 308 K

condition_3: pH: 7.0; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
COSYnot availablenot availablenot available

Software:

FELIX v97 -

NMR spectrometers:

  • Varian Inova 600 MHz
  • Bruker DMX 600 MHz