BMRB Entry 6410

Title:
1H, 15N, and 13C resonance assignments of reduced form of poplar glutaredoxin CxxC1 from Populus tremula tremuloides
Deposition date:
2004-12-04
Original release date:
2005-12-15
Authors:
Feng, Yingang; Rouhier, Nicolas; Jacquot, Jean-Pierre; Xia, Bin
Citation:

Citation: Feng, Yingang; Rouhier, Nicolas; Jacquot, Jean-Pierre; Xia, Bin. "Letter to the Editor: 1H, 15N, and 13C resonance assignments of reduced glutaredoxin C1 from Populus tremula x tremuloides"  J. Biomol. NMR 31, 263-264 (2005).

Assembly members:

Assembly members:
grxcxxc1, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Poplar   Taxonomy ID: 47664   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Populus tremula tremuloides

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Experimental source:

Natural source:   Common Name: Poplar   Taxonomy ID: 47664   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Populus tremula tremuloides

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts514
1H chemical shifts817
15N chemical shifts127

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Poplar Grx CxxC11

Entities:

Entity 1, Poplar Grx CxxC1 117 residues - Formula weight is not available

1   METALASERLYSGLNGLULEUASPALAALA
2   LEULYSLYSALALYSGLULEUALASERSER
3   ALAPROVALVALVALPHESERLYSTHRTYR
4   CYSGLYTYRCYSASNARGVALLYSGLNLEU
5   LEUTHRGLNVALGLYALASERTYRLYSVAL
6   VALGLULEUASPGLULEUSERASPGLYSER
7   GLNLEUGLNSERALALEUALAHISTRPTHR
8   GLYARGGLYTHRVALPROASNVALPHEILE
9   GLYGLYLYSGLNILEGLYGLYCYSASPTHR
10   VALVALGLULYSHISGLNARGASNGLULEU
11   LEUPROLEULEUGLNASPALAALAALATHR
12   ALALYSASNPROALAGLNLEU

Samples:

sample_1: grxcxxc1, [U-15N; U-13C], 1.1 mM; DTT 40 mM; DSS 0.01%; NaN3 0.01%; PBS 40 mM

conditions_1: pH: 6.4; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
HBHA(CBCA)(CO)NHsample_1not availableconditions_1
1H-13C HSQCsample_1not availableconditions_1
H(C)CH-TOCSYsample_1not availableconditions_1
(H)CCH-TOCSYsample_1not availableconditions_1
H(C)CH-COSYsample_1not availableconditions_1
(H)CCH-COSYsample_1not availableconditions_1
(HB)CB(CGCD)HDsample_1not availableconditions_1
(HB)CB(CGCDCE)HEsample_1not availableconditions_1
1H-13C NOESY-HSQC(optimized for aromatic region)sample_1not availableconditions_1
1H-15N TOCSY-HSQCsample_1not availableconditions_1
H(CCO)NHsample_1not availableconditions_1
(H)C(CO)NHsample_1not availableconditions_1
1H-15N NOESY-HSQCsample_1not availableconditions_1
1H-13C NOESY-HSQCsample_1not availableconditions_1

Software:

NMRPipe v2.3 -

NMRView v5.2.2 -

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB AAV73806

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts