BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6404

Title: HN, N, CA, CB Chemical Shift Assignments for the CID domain of Pcf11

Deposition date: 2004-10-25 Original release date: 2005-07-26

Authors: Hollingworth, David; Frenkiel, Thomas; Kelly, Geoff; Noble, Christian; Taylor, Ian; Ramos, Andres

Citation: Hollingworth, David; Kelly, Geoff; Frenkiel, Thomas; Noble, Christian; Taylor, Ian; Ramos, Andres. "Backbone assignment of PCF11 CTD binding domain"  J. Biomol. NMR 31, 363-363 (2005).

Assembly members:
Pcf11 CID, polymer, 142 residues, Formula weight is not available

Natural source:   Common Name: brewer's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomices cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Pcf11 CID: MDHDTEVIVKDFNSILEELT FNSRPIITTLTKLAEENISC AQYFVDAIESRIEKCMPKQK LYAFYALDSICKNVGSPYTI YFSRNLFNLYKRTYLLVDNT TRTKLINMFKLWLNPNDTGL PLFEGSALEKIEQFLIKASA LH

Data sets:
Data typeCount
1H chemical shifts130
15N chemical shifts130
13C chemical shifts258

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pcf11 CID1

Entities:

Entity 1, Pcf11 CID 142 residues - Formula weight is not available

1   METASPHISASPTHRGLUVALILEVALLYS
2   ASPPHEASNSERILELEUGLUGLULEUTHR
3   PHEASNSERARGPROILEILETHRTHRLEU
4   THRLYSLEUALAGLUGLUASNILESERCYS
5   ALAGLNTYRPHEVALASPALAILEGLUSER
6   ARGILEGLULYSCYSMETPROLYSGLNLYS
7   LEUTYRALAPHETYRALALEUASPSERILE
8   CYSLYSASNVALGLYSERPROTYRTHRILE
9   TYRPHESERARGASNLEUPHEASNLEUTYR
10   LYSARGTHRTYRLEULEUVALASPASNTHR
11   THRARGTHRLYSLEUILEASNMETPHELYS
12   LEUTRPLEUASNPROASNASPTHRGLYLEU
13   PROLEUPHEGLUGLYSERALALEUGLULYS
14   ILEGLUGLNPHELEUILELYSALASERALA
15   LEUHIS

Samples:

sample_1: Pcf11 CID, [U-13C; U-15N; U-2H], 0.8 mM

sample_2: Pcf11 CID, [U-13C; U-15N], 0.8 mM

sample_3: Pcf11 CID, [U-15N], 0.8 mM

conditions_1: pH: 7.4; temperature: 300 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
TROSY-HNCACBnot availablenot availableconditions_1
TROSY-HNCAnot availablenot availableconditions_1
15N-edited NOESYnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 800 MHz

Related Database Links:

PDB
DBJ GAA22455
EMBL CAA88508 CAY78728
GB AHY75209 EDN60562 EDV08088 EDZ73030 EEU05243
REF NP_010514
SP P39081
TPG DAA12070

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts