BMRB Entry 6401

Title:
NMR assignment of R55F mutant of Mason-Pfizer monkey virus matrix protein
Deposition date:
2004-11-24
Original release date:
2005-07-26
Authors:
Veverka, Vaclav; Vlach, Jiri; Lipov, Jan; Rumlova, Michaela; Ruml, Tomas; Hrabal, Richard
Citation:

Citation: Vlach, Jiri; Lipov, Jan; Veverka, V.; Rumlova, M.; Ruml, Tomas; Hrabal, Richard. "Letter to the Editor: Assignment of 1H, 13C, and 15N resonances of WT matrix protein and its R55F mutant from Mason-Pfizer monkey virus"  J. Biomol. NMR 31, 381-382 (2005).

Assembly members:

Assembly members:
R55F, polymer, 100 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mason-Pfizer monkey virus   Taxonomy ID: 11855   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betaretrovirus Mason-Pfizer monkey virus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts297
1H chemical shifts552
15N chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1R55F1

Entities:

Entity 1, R55F 100 residues - Formula weight is not available

1   METGLYGLNGLULEUSERGLNHISGLUARG
2   TYRVALGLUGLNLEULYSGLNALALEULYS
3   THRARGGLYVALLYSVALLYSTYRALAASP
4   LEULEULYSPHEPHEASPPHEVALLYSASP
5   THRCYSPROTRPPHEPROGLNGLUGLYTHR
6   ILEASPILELYSPHETRPARGARGVALGLY
7   ASPCYSPHEGLNASPTYRTYRASNTHRPHE
8   GLYPROGLULYSVALPROVALTHRALAPHE
9   SERTYRTRPASNLEUILELYSGLULEUILE
10   ASPLYSLYSGLUVALASNPROGLNVALMET

Samples:

sample_1: R55F, [U-13C; U-15N], 1 mM; NaH2PO4/Na2HPO4 100 mM; NaCl 200 mM; D2O 10%; DTT 5%

conditions_1: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_1
1H13C HSQCsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
CC-TOCSYsample_1not availableconditions_1
HcCH-COSYsample_1not availableconditions_1
3D 15N-edited NOESYsample_1not availableconditions_1
3D 13C-edited NOESYsample_1not availableconditions_1
4D 13C,15N-edited NOESYsample_1not availableconditions_1

Software:

NMRPipe v2.3 -

Sparky v3.110 -

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

BMRB 18282 25087 6400
PDB
GB AAA47710 AAA47730 AAC82573 AAC82574 AAC82576
PIR FOLJSA
REF NP_056891 NP_056892 NP_056893 NP_954557 NP_954565
SP P04022 P07567
AlphaFold P04022 P07567

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks