BMRB Entry 6399

Name: 1H, 13C and 15N resonance assignments for the 24 kDa TPR domain in p67phox
Published: 2005-07-26

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PDB ID: 1hh8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6399
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignments for the 24 kDa TPR domain in p67phox

Deposition date:

2004-11-23

Original release date:

2005-07-26

Authors:

Yoshida, Shinichi; Ogura, Kenji; Yokochi, Masashi; Yuzawa, Satoru; Horiuchi, Masataka; Morioka, Hiroshi; Sumimoto, Hideki; Inagaki, Fuyuhiko

Citation:

Citation: Yoshida, Shinichi; Ogura, Kenji; Yokochi, Masashi; Yuzawa, Satoru; Horiuchi, Masataka; Morioka, Hiroshi; Sumimoto, Hideki; Inagaki, Fuyuhiko. "1H, 13C and 15N resonance assignments of the backbone and methyl groups of the 24 kDa tetratricopeptide repeat domain in p67phox" J. Biomol. NMR 32, 176-176 (2005).

Assembly members:

Assembly members:
TPR, polymer, 208 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pPROEX-HTb

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TPR: GAMGSMSLVEAISLWNEGVL AADKKDWKGALDAFSAVQDP HSRICFNIGCMYTILKNMTE AEKAFTRSINRDKHLAVAYF QRGMLYYQTEKYDLAIKDLK EALIQLRGNQLIDYKILGLQ FKLFACEVLYNIAFMYAKKE EWKKAEEQLALATSMKSEPR HSKIDKAMECVWKQKLYEPV VIPVGKLFRPNERQVAQLAK KDYLGKAT

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	675
1H chemical shifts	457
15N chemical shifts	215

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TPR domain in p67phox	1

Entities:

Entity 1, TPR domain in p67phox 208 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

SER

MET

SER

LEU

VAL

GLU

2

ALA

ILE

SER

LEU

TRP

ASN

GLU

GLY

VAL

LEU

3

ALA

ASP

LYS

ASP

TRP

LYS

GLY

ALA

4

LEU

ASP

ALA

PHE

SER

ALA

VAL

GLN

ASP

PRO

5

HIS

SER

ARG

ILE

CYS

PHE

ASN

ILE

GLY

CYS

6

MET

TYR

THR

ILE

LEU

LYS

ASN

MET

THR

GLU

7

ALA

GLU

LYS

ALA

PHE

THR

ARG

SER

ILE

ASN

8

ARG

ASP

LYS

HIS

LEU

ALA

VAL

ALA

TYR

PHE

9

GLN

ARG

GLY

MET

LEU

TYR

GLN

THR

GLU

10

LYS

TYR

ASP

LEU

ALA

ILE

LYS

ASP

LEU

LYS

11

GLU

ALA

LEU

ILE

GLN

LEU

ARG

GLY

ASN

GLN

12

LEU

ILE

ASP

TYR

LYS

ILE

LEU

GLY

LEU

GLN

13

PHE

LYS

LEU

PHE

ALA

CYS

GLU

VAL

LEU

TYR

14

ASN

ILE

ALA

PHE

MET

TYR

ALA

LYS

GLU

15

GLU

TRP

LYS

ALA

GLU

GLN

LEU

ALA

16

LEU

ALA

THR

SER

MET

LYS

SER

GLU

PRO

ARG

17

HIS

SER

LYS

ILE

ASP

LYS

ALA

MET

GLU

CYS

18

VAL

TRP

LYS

GLN

LYS

LEU

TYR

GLU

PRO

VAL

19

VAL

ILE

PRO

VAL

GLY

LYS

LEU

PHE

ARG

PRO

20

ASN

GLU

ARG

GLN

VAL

ALA

GLN

LEU

ALA

LYS

21

LYS

ASP

TYR

LEU

GLY

LYS

ALA

THR

Samples:

sample_1: TPR, [U-80%(fractional) 2H; U-98% 13C; U-98% 15N], 0.8 mM; Na-Pi 50 mM; NaCl 150 mM; DTT 1 mM; MgCl2 10 mM; EDTA 0.5 mM; H2O 90%; D2O 10%

sample_2: TPR, [NA-Val, Leu and Ile(d1) methyl; U-98% 2H; U-98% 13C; U-98% 15N], 0.7 mM; Na-Pi 50 mM; NaCl 150 mM; DTT 1 mM; MgCl2 10 mM; EDTA 0.5 mM; H2O 90%; D2O 10%

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	not available	not available	conditions_1
2D 1H-13C CT-HSQC	not available	not available	conditions_1
HNCO	not available	not available	conditions_1
HN(CA)CO	not available	not available	conditions_1
HNCA	not available	not available	conditions_1
HN(CO)CA	not available	not available	conditions_1
HN(CA)CB	not available	not available	conditions_1
HN(COCA)CB	not available	not available	conditions_1
C(CO)NH	not available	not available	conditions_1
H(CCO)NH	not available	not available	conditions_1
H(C)CH-TOCSY	not available	not available	conditions_1

Software:

NMRPipe/NMRDraw - processing, peak-pick

Olivia, Yokochi Masashi - assignment works

NMR spectrometers:

Varian Unity-Plus or AS 600 MHz
Varian AS 500 MHz

PDB	1E96 1HH8 1WM5
DBJ	BAD92884 BAG35548 BAJ20290
EMBL	CAH92976
GB	AAA36379 AAB60320 AAH01606 AAM89263 AAP36107
REF	NP_000424 NP_001121123 NP_001127613 XP_001114105 XP_003264493
SP	P19878
AlphaFold	P19878