BMRB Entry 6396

Name: Chemical shift Assignment for mouse Roadblock/LC7 domain BC029172
Published: 2007-01-29

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PDB ID: 1y4o
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6396
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shift Assignment for mouse Roadblock/LC7 domain BC029172

Deposition date:

2004-11-22

Original release date:

2007-01-29

Authors:

Song, Jikui; Vinarov, Dmitriy; Tyler, Ejan; Shahan, Mark; Loushin Newman, Carrie; Tyler, Robert; Lee, Min; Markley, John

Citation:

Citation: Song, Jikui; Tyler, Robert; Lee, Min; Tyler, Ejan; Markley, John. "Solution structure of isoform 1 of Roadblock/LC7, a light chain in the dynein complex" J. Mol. Biol. 354, 1043-1051 (2005).
PubMed: 16289575

Assembly members:

Assembly members:
BC029172, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BC029172: GHHHHHHLEAEVEETLKRLQ SQKGVQGIIVVNTEGIPIKS TMDNPTTTQYANLMHNFILK ARSTVREIDPQNDLTFLRIR SKKNEIMVAPDKDYFLIVIQ NPTE

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	399
1H chemical shifts	643
15N chemical shifts	108

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BC029172	1

Entities:

Entity 1, BC029172 104 residues - Formula weight is not available

1

GLY

HIS

LEU

GLU

ALA

2

GLU

VAL

GLU

THR

LEU

LYS

ARG

LEU

GLN

3

SER

GLN

LYS

GLY

VAL

GLN

GLY

ILE

VAL

4

VAL

ASN

THR

GLU

GLY

ILE

PRO

ILE

LYS

SER

5

THR

MET

ASP

ASN

PRO

THR

GLN

TYR

6

ALA

ASN

LEU

MET

HIS

ASN

PHE

ILE

LEU

LYS

7

ALA

ARG

SER

THR

VAL

ARG

GLU

ILE

ASP

PRO

8

GLN

ASN

ASP

LEU

THR

PHE

LEU

ARG

ILE

ARG

9

SER

LYS

ASN

GLU

ILE

MET

VAL

ALA

PRO

10

ASP

LYS

ASP

TYR

PHE

LEU

ILE

VAL

ILE

GLN

11

ASN

PRO

THR

GLU

Samples:

sample_1: BC029172, [U-15N; U-13C], 1 mM; Sodium Chloride 100 mM; Sodium Acetate 10 mM

conditions_1: pH: 5.0; temperature: 298 K; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-NOESY	sample_1	not available	conditions_1
3D 13C-NOESY	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Varian Inova 600 MHz

PDB	1Y4O
DBJ	BAB22038 BAB22788 BAB25768 BAE38944
GB	AAC25580 AAH58437 AAH99423 AAI02139 AAI25611
REF	NP_001029614 NP_001177109 NP_001239348 NP_001278037 NP_080223
SP	P62627 P62628 Q3T140
TPG	DAA23215
AlphaFold	P62627 P62628 Q3T140