BMRB Entry 6395

Title:
1H, 13C, and 15N Chemical Shift Assignments for the Cis and Trans Isomer of the Beta Domain of Bacillus stearothermophilus DivIB
Deposition date:
2004-11-17
Original release date:
2005-08-01
Authors:
Robson, Scott; Gorbatyuk, Vitaliy; Maciejewski, Mark; King, Glenn
Citation:

Citation: Robson, Scott; Gorbatyuk, Vitaliy; Maciejewski, Mark; King, Glenn. "Letter to the Editor: Backbone and side-chain 1H, 15N, and 13C assignments for the beta domain of the bacterial cell division protein DivIB"  J. Biomol. NMR 31, 261-262 (2005).
PubMed: 15803402

Assembly members:

Assembly members:
DivIB beta domain, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus stearothermophilus   Taxonomy ID: 1422   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus stearothermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-2T

Data sets:
Data typeCount
13C chemical shifts973
1H chemical shifts1692
15N chemical shifts242

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DivIB beta domain, trans isomer1
2DivIB beta domain, cis isomer1

Entities:

Entity 1, DivIB beta domain, trans isomer 119 residues - Formula weight is not available

1   GLYSERGLUTRPARGARGILEALATYRVAL
2   TYRASPARGGLNTHRPHEPHEPROLEULEU
3   GLUASNGLYARGLEULEULYSGLNGLUGLY
4   THRLYSTHRALAPROSERASPALAPROVAL
5   LEUVALGLYTRPLYSASPGLYASPALAILE
6   ALAGLUMETTHRGLYGLNLEUALAGLULEU
7   PROALAALAVALLEUGLYALAMETSERGLU
8   ILEHISTYRLYSPROTHRARGGLUTYRGLU
9   ASPARGVALILEVALTYRMETASNASPGLY
10   TYRGLUVALSERALATHRILEARGGLNPHE
11   ALAASPLYSLEUSERHISTYRPROALAILE
12   ALAALAALALEUASPARGASNVALLYS

Samples:

sample_1: DivIB beta domain, [U-13C; U-15N], 1 mM; Sodium phosphate 10 mM; NaCl 150 mM; Sodium azide 1 mM; EDTA 10 um; AEBSF 10 um; D2O 7 % v/v

sample_2: DivIB beta domain, [U-15N], 1 mM; Sodium phosphate 10 mM; NaCl 150 mM; Sodium azide 1 mM; EDTA 10 um; AEBSF 10 um; D2O 7 % v/v

conditions_1: pH: 6.0; temperature: 308 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 15N-edited HSQCnot availablenot availableconditions_1
3D CBCA(CO)HNnot availablenot availableconditions_1
3D HNCACBnot availablenot availableconditions_1
3D (H)CC(CO)HN-TOCSYnot availablenot availableconditions_1
3D H(CC)(CO)HN-TOCSYnot availablenot availableconditions_1
3D HCCH-TOCSYnot availablenot availableconditions_1
3D 15N-edited NOESY-HSQCnot availablenot availableconditions_1
3D 13C-edited NOESY-HSQCnot availablenot availableconditions_1
2D 1H-1H NOESYnot availablenot availableconditions_1
2D 1H-1H TOCSYnot availablenot availableconditions_1
3D HNHAnot availablenot availableconditions_1
3D HNHBnot availablenot availableconditions_1
3D HNCOnot availablenot availableconditions_1
3D HNCACOnot availablenot availableconditions_1

Software:

nmrPipe v1.0 - NMR data processing

XEASY v1..3.13 - resonance assignment, integration

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

PDB
DBJ BAD75405 GAD14544 GAJ59556
EMBL CAJ75583
GB ACX78517 ADI27411 ADU93491 AEV18620 AGE21595
REF WP_011230620 WP_013145845 WP_013523339 WP_014195375 WP_015374358
SP Q5L0X5
AlphaFold Q5L0X5 Q5L0X5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks