BMRB Entry 6390

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6390
MolProbity Validation Chart

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Title:
NMR assignment of the R-module from the Azotobacter vinelandii mannuronan C5-epimerase AlgE4
Deposition date:
2004-11-15
Original release date:
2005-04-08
Authors:
Aachmann, Finn; Svanem, Britt Iren; Valla, Svein; Petersen, Steffen; Wimmer, Reinhard
Citation:

Citation: Aachmann, Finn; Svanem, Britt Iren; Valla, Svein; Petersen, Steffen; Wimmer, Reinhard. "NMR assignment of the R-module from the Azotobacter vinelandii mannuronan C5-epimerase AlgE4"  J. Biomol. NMR 31, 259-259 (2005).

Assembly members:

Assembly members:
R-module, polymer, 167 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Azotobacter vinelandii   Taxonomy ID: 354   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Azotobacter vinelandii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTYB11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
R-module: GSDGEPLVGGDTDDQLQGGS GADRLDGGAGDDILDGGAGR DRLSGGAGADTFVFSAREDS YRTDTAVFNDLILDFEASED RIDLSALGFSGLGDGYGGTL LLKTNAEGTRTYLKSFEADA EGRRFEVALDGDHTGDLSAA NVVFAATGTTTELEVLGDSG TQAGAIV

Data sets:
Data typeCount
13C chemical shifts571
1H chemical shifts924
15N chemical shifts166

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1R-module1
2CALCIUM (II) ION2

Entities:

Entity 1, R-module 167 residues - Formula weight is not available

1   GLYSERASPGLYGLUPROLEUVALGLYGLY
2   ASPTHRASPASPGLNLEUGLNGLYGLYSER
3   GLYALAASPARGLEUASPGLYGLYALAGLY
4   ASPASPILELEUASPGLYGLYALAGLYARG
5   ASPARGLEUSERGLYGLYALAGLYALAASP
6   THRPHEVALPHESERALAARGGLUASPSER
7   TYRARGTHRASPTHRALAVALPHEASNASP
8   LEUILELEUASPPHEGLUALASERGLUASP
9   ARGILEASPLEUSERALALEUGLYPHESER
10   GLYLEUGLYASPGLYTYRGLYGLYTHRLEU
11   LEULEULYSTHRASNALAGLUGLYTHRARG
12   THRTYRLEULYSSERPHEGLUALAASPALA
13   GLUGLYARGARGPHEGLUVALALALEUASP
14   GLYASPHISTHRGLYASPLEUSERALAALA
15   ASNVALVALPHEALAALATHRGLYTHRTHR
16   THRGLULEUGLUVALLEUGLYASPSERGLY
17   THRGLNALAGLYALAILEVAL

Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.

1   CA

Samples:

sample_1: R-module, [U-99% 13C; U-99% 15N], 1.6 mM; CALCIUM (II) ION 1.6 mM; CaCl2 50 mM; Na-HEPES 20 mM

sample_2: R-module, [U-99% 13C; U-99% 15N], 0.8 – 1.1 mM; CALCIUM (II) ION0.8 – 1.1 mM; CaCl2 50 mM; Na-HEPES 20 mM

ex-rm: pH: 6.9; temperature: 298 K; ionic strength: 0.17 M

Experiments:

NameSampleSample stateSample conditions
Aromatic side-chain atoms were assigned from a series of 2D H(CC)H-COSY, TOCSYnot availablenot availableex-rm
and NOESY experiments optimized for aromatic signals.not availablenot availableex-rm
Due to the high content of Glycine and Alanine residues, MUSIC spectra havenot availablenot availableex-rm
been very helpful to identify Gly/Ala residues and residues following afternot availablenot availableex-rm
Gly/Ala.not availablenot availableex-rm

Software:

XEASY v1.3.13 -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
GB AAA87310 ACO81204 ADG44967 AGK13234 AGK17521
REF WP_012703557 YP_002802179 YP_007895988 YP_007900994
SP Q44493
AlphaFold Q44493

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