BMRB Entry 6374

Click here to enlarge.

PDB ID: 1xwh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6374
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

NMR STRUCTURE OF THE FIRST PHD FINGER OF AUTOIMMUNE REGULATOR PROTEIN (AIRE1): INSIGHTS INTO APECED

Deposition date:

2004-11-01

Original release date:

2005-02-10

Authors:

Bottomley, Matthew; Stier, Gunter; Krasotkina, Julya; Legube, Gaelle; Simon, Bernd; Akhtar, Asifa; Sattler, Michael; Musco, Giovanna

Citation:

Citation: Bottomley, Matthew; Stier, Gunter; Pennacchini, D.; Legube, Gaelle; Simon, Bernd; Akhtar, Asifa; Sattler, Michael; Musco, Giovanna. "NMR structure of the first PHD finger of autoimmune regulator protein (AIRE1). Insights into autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) disease" J. Biol. Chem. 280, 11505-11512 (2005).
PubMed: 15649886

Assembly members:

Assembly members:
first PHD finger of AIRE1, polymer, 66 residues, 7138 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
first PHD finger of AIRE1: GAMAQKNEDECAVCRDGGEL ICCDGCPRAFHLACLSPPLR EIPSGTWRCSSCLQATVQEV QPRAEE

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1
- chemical_shift_set_2
RDCs
- residual_dipolar_couplings_set_1

Data type	Count
13C chemical shifts	201
15N chemical shifts	61
1H chemical shifts	649
residual dipolar couplings	42

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AIRE1-PHD1 monomer	1
2	ZINC (II) ION, 1	2
3	ZINC (II) ION, 2	2

Entities:

Entity 1, AIRE1-PHD1 monomer 66 residues - 7138 Da.

1

GLY

ALA

MET

ALA

GLN

LYS

ASN

GLU

ASP

GLU

2

CYS

ALA

VAL

CYS

ARG

ASP

GLY

GLU

LEU

3

ILE

CYS

ASP

GLY

CYS

PRO

ARG

ALA

PHE

4

HIS

LEU

ALA

CYS

LEU

SER

PRO

LEU

ARG

5

GLU

ILE

PRO

SER

GLY

THR

TRP

ARG

CYS

SER

6

SER

CYS

LEU

GLN

ALA

THR

VAL

GLN

GLU

VAL

7

GLN

PRO

ARG

ALA

GLU

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: first PHD finger of AIRE1, [U-13C; U-15N], 1 mM; NaCl 150 mM; phosphate 20 mM; DTT 5 mM

Ex-cond_1: ionic strength: 0.15 M; pH*: 6.3; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_1	not available	Ex-cond_1
CBCA(CO)NH	sample_1	not available	Ex-cond_1
CBCANH	sample_1	not available	Ex-cond_1
3D HCCONH-TOCSY	sample_1	not available	Ex-cond_1
CCONH-TOCSY	sample_1	not available	Ex-cond_1
HCCH-TOCSY	sample_1	not available	Ex-cond_1
2D 1H-15N HMQC	sample_1	not available	Ex-cond_1

Software:

No software information available

NMR spectrometers:

Bruker DRX 500 MHz
Bruker DRX 600 MHz