BMRB Entry 6367

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6367
MolProbity Validation Chart

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Title:
1H, 13C, and 15N Chemical Shift Assignments of E.Coli Protein yhgG: The Northeast Structural Genomics Consortium Target ET95
Deposition date:
2004-10-27
Original release date:
2008-08-28
Authors:
Shen, Yang; Acton, Thomas; Atreya, Hanudatta; Ma, LiChung; Liu, Gaohua; Xiao, Rong; Montelione, Gaetano; Szyperski, Thomas
Citation:

Citation: Liu, Gaohua; Shen, Yang; Atreya, Hanudatta; Parish, David; Shao, Ying; Sukumaran, Dinesh; Xiao, Rong; Yee, Adelinda; Lemak, Alexander; Bhattacharya, Aneerban; Acton, Thomas; Arrowsmith, Cheryl; Montelione, Gaetano; Szyperski, Thomas. "NMR data collection and analysis protocol for high-throughput protein structure determination"  Proc. Natl. Acad. Sci. U.S.A. 102, 10487-10492 (2005).
PubMed: 16027363

Assembly members:

Assembly members:
Hypothetical protein yhgG, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. Coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: Not applicable   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hypothetical protein yhgG: MASLIQVRDLLALRGRMEAA QISQTLNTPQPMINAMLQQL ESMGKAVRIQEEPDGCLSGS CKSCPEGKACLREWWALR

Data sets:
Data typeCount
1H chemical shifts533
13C chemical shifts321
15N chemical shifts80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Hypothetical protein yhgG1

Entities:

Entity 1, Hypothetical protein yhgG 78 residues - Formula weight is not available

1   METALASERLEUILEGLNVALARGASPLEU
2   LEUALALEUARGGLYARGMETGLUALAALA
3   GLNILESERGLNTHRLEUASNTHRPROGLN
4   PROMETILEASNALAMETLEUGLNGLNLEU
5   GLUSERMETGLYLYSALAVALARGILEGLN
6   GLUGLUPROASPGLYCYSLEUSERGLYSER
7   CYSLYSSERCYSPROGLUGLYLYSALACYS
8   LEUARGGLUTRPTRPALALEUARG

Samples:

sample_1: Hypothetical protein yhgG, [U-13C; U-15N], 1.13 mM

sample_conditions: pH: 6.5; temperature: 298 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
GFT (4,3)D HNNCABCAnot availablenot availablenot available
GFT (4,3)D CABCA(CO)NHNnot availablenot availablenot available
GFT (5,2)D HACACONHNnot availablenot availablenot available
GFT (4,3)D HCCHnot availablenot availablenot available
SIMULTANEOUS HETERONUCLEAR RESOLVED [1H,1H]-NOESYnot availablenot availablenot available

Software:

PROSA v6.0 -

XEASY v1.3.1.3 -

AUTOASSIGN v1.13.2 -

NMR spectrometers:

  • VARIAN Inova 750 MHz
  • VARIAN Inova 600 MHz

Related Database Links:

PDB
DBJ BAB37675 BAE77881 BAG79200 BAI27668 BAI32838
EMBL CAP77856 CAQ33731 CAQ90856 CAR00353 CAR05011
GB AAA58208 AAC76435 AAG58511 AAN44890 AAN82625
REF NP_289950 NP_312279 NP_417869 NP_709183 NP_756051
SP A7ZST9 A8A5L8 B1IP55 B1LHL0 B1X757
AlphaFold A7ZST9 A8A5L8 B1IP55 B1LHL0 B1X757

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks