BMRB Entry 6364

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6364

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C 15N chemical shift assignment of Pyrococcus furiosus Protein PF0470: The Northeast Structural Genomics Consortium Target PfR14

Deposition date:

2004-10-27

Original release date:

2008-08-28

Authors:

Liu, Gaohua; Xiao, Rong; Parish, David; Ma, Lichung; Sukumaran, Dinesh; Acton, Thomas; Montelione, Gaetano; Szyperski, Thomas

Citation:

Citation: Liu, Gaohua; Shen, Yang; Atreya, Hanudatta; Parish, David; Shao, Ying; Sukumaran, Dinesh; Xiao, Rong; Yee, Adelinda; Lemak, Alexander; Bhattacharya, Aneerban; Acton, Thomas; Arrowsmith, Cheryl; Montelione, Gaetano; Szyperski, Thomas. "NMR data collection and analysis protocol for high-throughput protein structure determination" Proc. Natl. Acad. Sci. U.S.A. 102, 10487-10492 (2005).
PubMed: 16027363

Assembly members:

Assembly members:
Protein PF0470, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Pyrococcus furiosus Taxonomy ID: 2261 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Protein PF0470: MKVYRLYLKDEYLEMVKSGK KRIEVRVAYPQLKDIKRGDK IIFNDLIPAEVVEVKKYETF RQVLREEPIDKIFPDKPSFE KALKRFHNMYPKWKEYRYGV LAIKFRVLGRDKE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts

Data type	Count
1H chemical shifts	815
13C chemical shifts	415
15N chemical shifts	90

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Protein PF0470	1

Entities:

Entity 1, Protein PF0470 113 residues - Formula weight is not available

1

MET

LYS

VAL

TYR

ARG

LEU

TYR

LEU

LYS

ASP

2

GLU

TYR

LEU

GLU

MET

VAL

LYS

SER

GLY

LYS

3

LYS

ARG

ILE

GLU

VAL

ARG

VAL

ALA

TYR

PRO

4

GLN

LEU

LYS

ASP

ILE

LYS

ARG

GLY

ASP

LYS

5

ILE

PHE

ASN

ASP

LEU

ILE

PRO

ALA

GLU

6

VAL

GLU

VAL

LYS

TYR

GLU

THR

PHE

7

ARG

GLN

VAL

LEU

ARG

GLU

PRO

ILE

ASP

8

LYS

ILE

PHE

PRO

ASP

LYS

PRO

SER

PHE

GLU

9

LYS

ALA

LEU

LYS

ARG

PHE

HIS

ASN

MET

TYR

10

PRO

LYS

TRP

LYS

GLU

TYR

ARG

TYR

GLY

VAL

11

LEU

ALA

ILE

LYS

PHE

ARG

VAL

LEU

GLY

ARG

12

ASP

LYS

GLU

Samples:

sample: Protein PF0470, [U-13C; U-15C], 1 mM; MES 20 mM; NaCl 100 mM; DTT 10 mM; CaCl2 5 mM; NaN3 0.02%; H2O 95%; D2O 5%

sample_conditions: temperature: 298 K; pH: 6.5; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
GFT (4,3)D HNNCabCa'	not available	not available	not available
GFT (4,3)D CabCa(CO)NHN'	not available	not available	not available
GFT (4,3)D HabCab(CO)NHN'	not available	not available	not available
GFT (4,3) HCCH'	not available	not available	not available
Simultaneous Heteronuclear Resolved NOESY	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Varian Inova 600 MHz
Varian Inova 750 MHz

PDB	1XNE
GB	AAL80594 AFN03264
REF	NP_578199 WP_011011587 YP_006491556