BMRB Entry 6353

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6353

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C resonance assignments of the BRCT Region of the large subunit of human Replication Factor C

Deposition date:

2004-10-14

Original release date:

2010-07-14

Authors:

Kobayashi, Masakazu; Siegal, Gregg

Citation:

Citation: Kobayashi, Masakazu; Siegal, Gregg. "Letter to the Editor: 1H, 15N and 13C resonance assignments of the BRCT Region of the large subunit of human Replication Factor C" J. Biomol. NMR 31, 183-184 (2005).

Assembly members:

Assembly members:
RFC p140 BRCT region, polymer, 106 residues, Formula weight is not available
self - annealing hairpin - dsDNA, polymer, 28 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Eschericia coli Vector: pLysS

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RFC p140 BRCT region: KRTNYQAYRSYLNREGPKAL GSKEIPKGAENCLEGLIFVI TGVLESIERDEAKSLIERYG GKVTGNVSKKTNYLVMGRDS GQSKSDKAAALGTKIIDEDG LLNLIR
self - annealing hairpin - dsDNA: CTCGAGGTCGTCATCGACCT CGAGATCA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts

Data type	Count
1H chemical shifts	748
13C chemical shifts	321
15N chemical shifts	113

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RFC p140 375-480 BRCT region	1
2	self - annealing hairpin - dsDNA	2

Entities:

Entity 1, RFC p140 375-480 BRCT region 106 residues - Formula weight is not available

1

LYS

ARG

THR

ASN

TYR

GLN

ALA

TYR

ARG

SER

2

TYR

LEU

ASN

ARG

GLU

GLY

PRO

LYS

ALA

LEU

3

GLY

SER

LYS

GLU

ILE

PRO

LYS

GLY

ALA

GLU

4

ASN

CYS

LEU

GLU

GLY

LEU

ILE

PHE

VAL

ILE

5

THR

GLY

VAL

LEU

GLU

SER

ILE

GLU

ARG

ASP

6

GLU

ALA

LYS

SER

LEU

ILE

GLU

ARG

TYR

GLY

7

GLY

LYS

VAL

THR

GLY

ASN

VAL

SER

LYS

8

THR

ASN

TYR

LEU

VAL

MET

GLY

ARG

ASP

SER

9

GLY

GLN

SER

LYS

SER

ASP

LYS

ALA

10

LEU

GLY

THR

LYS

ILE

ASP

GLU

ASP

GLY

11

LEU

ASN

LEU

ILE

ARG

Entity 2, self - annealing hairpin - dsDNA 28 residues - Formula weight is not available

1

DC

DT

DC

DG

DA

DG

DT

DC

DG

2

DT

DC

DA

DT

DC

DG

DA

DC

DT

3

DC

DG

DA

DG

DA

DT

DC

DA

Samples:

sample_1: RFC p140 BRCT region, [U-15N; U-13C], 0.5 mM; self - annealing hairpin - dsDNA 0.5 mM; Tris-HCl 20 mM; NaCl 5 mM; DTT 1 mM

sample_conditions: pH: 7.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	sample_1	not available	sample_conditions

Software:

XEASY -

NMR spectrometers:

Bruker DMX 600 MHz

PDB	2EBU 2K6G 2K7F
DBJ	BAE88328 BAF84301 BAG10592
EMBL	CAA49475 CAA51260 CAA80355
GB	AAA16121 AAA21643 AAA79698 AAA81558 AAB60452
REF	NP_001191676 NP_002904 NP_035388 XP_001091287 XP_001140765
SP	P35251 P35601
AlphaFold	P35251 P35601