BMRB Entry 6346
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6346
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
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Citation: Tong, Yufeng; Buck, Matthias. "Letter to the Editor: 1H, 15N and 13C Resonance assignments and secondary
structure determination reveal that the minimal Rac1 GTPase binding domain
of plexin-B1 has a ubiquitin fold" J. Biomol. NMR 31, 369-370 (2005).
PubMed: 15929008
Assembly members:
plexin-B1 RBD, polymer, 122 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
plexin-B1 RBD: KKDVEYRPLTLNALLAVGPG
AGEAQGVPVKVLDCDTISQA
KEKMLDQLYKGVPLTQRPDP
RTLDVEWRSGVAGHLILSDE
DVTSEVQGLFRRLNTLQHYK
VPDGATVALVPCLTKHVLRE
NQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 517 |
| 15N chemical shifts | 123 |
| 1H chemical shifts | 840 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | plexin-B1 RBD-W1830F | 1 |
Entities:
Entity 1, plexin-B1 RBD-W1830F 122 residues - Formula weight is not available
| 1 | LYS | LYS | ASP | VAL | GLU | TYR | ARG | PRO | LEU | THR | ||||
| 2 | LEU | ASN | ALA | LEU | LEU | ALA | VAL | GLY | PRO | GLY | ||||
| 3 | ALA | GLY | GLU | ALA | GLN | GLY | VAL | PRO | VAL | LYS | ||||
| 4 | VAL | LEU | ASP | CYS | ASP | THR | ILE | SER | GLN | ALA | ||||
| 5 | LYS | GLU | LYS | MET | LEU | ASP | GLN | LEU | TYR | LYS | ||||
| 6 | GLY | VAL | PRO | LEU | THR | GLN | ARG | PRO | ASP | PRO | ||||
| 7 | ARG | THR | LEU | ASP | VAL | GLU | TRP | ARG | SER | GLY | ||||
| 8 | VAL | ALA | GLY | HIS | LEU | ILE | LEU | SER | ASP | GLU | ||||
| 9 | ASP | VAL | THR | SER | GLU | VAL | GLN | GLY | LEU | PHE | ||||
| 10 | ARG | ARG | LEU | ASN | THR | LEU | GLN | HIS | TYR | LYS | ||||
| 11 | VAL | PRO | ASP | GLY | ALA | THR | VAL | ALA | LEU | VAL | ||||
| 12 | PRO | CYS | LEU | THR | LYS | HIS | VAL | LEU | ARG | GLU | ||||
| 13 | ASN | GLN |
Samples:
sample_1: plexin-B1 RBD, [U-13C; U-15N], 1.2 mM
sample_2: plexin-B1 RBD, [U-13C], 2 mM
condition_1: pH: 6.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C CT-HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNHA | not available | not available | not available |
| H(CCCO)NH | not available | not available | not available |
| CC(CO)NH | not available | not available | not available |
| HCCH-TOSY | not available | not available | not available |
| RD-HBCB(CGCD)HD | not available | not available | not available |
| 1H-TOCSY-relayed RD-HCH-COSY | not available | not available | not available |
| NOESY-15N-HSQC | not available | not available | not available |
| NOESY-13C-HSQC | not available | not available | not available |
Software:
NMRView v5.2.2 - Spectral Analysis
NMRPipe v2.3 - Data processing
NMR spectrometers:
- Varian INOVA 600 MHz
- Bruker ICE 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA23703 BAG10332 BAG52423 BAG52582 |
| EMBL | CAB56222 CAB57277 |
| GB | AAI46794 EAW64864 EAW64865 EAW64866 |
| REF | NP_001123554 NP_002664 XP_003257054 XP_003926345 XP_004034120 |
| SP | O43157 |
| AlphaFold | O43157 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks