BMRB Entry 6343

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6343
MolProbity Validation Chart

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Title:
Backbone and side chain 1H, 13C, and 15N chemical shift assignments of PA1324 (21-170): Northeast Structural Genomics Consortium Target PaP1
Deposition date:
2004-10-07
Original release date:
2004-11-29
Authors:
Cort, John; Kennedy, Michael; Ni, Shuisong
Citation:

Citation: Cort, John; Kennedy, Michael; Ni, Shuisong. "Chemical shift assignments of PA1324 (21-170)"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
PA1324 (21-170), polymer, 170 residues, 18511.4 Da.

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PA1324 (21-170): GSSHHHHHHSSGLVPRGSHM ASNPNDLPDFPEHEYAATQQ VGGGVINGDLYLTSASGAIQ KGTNTKVALEPATSYMKAYY AKFGNLDAAKRDPDVQPPVL DPRRATYVREATTDQNGRFD FDHIPNGTYYISSELTWSAQ SDGKTITEGGTVTKLVTVSG SQPQKVLLTR

Data sets:
Data typeCount
13C chemical shifts614
15N chemical shifts154
1H chemical shifts946

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PA1324 (21-170)1

Entities:

Entity 1, PA1324 (21-170) 170 residues - 18511.4 Da.

1   GLYSERSERHISHISHISHISHISHISSER
2   SERGLYLEUVALPROARGGLYSERHISMET
3   ALASERASNPROASNASPLEUPROASPPHE
4   PROGLUHISGLUTYRALAALATHRGLNGLN
5   VALGLYGLYGLYVALILEASNGLYASPLEU
6   TYRLEUTHRSERALASERGLYALAILEGLN
7   LYSGLYTHRASNTHRLYSVALALALEUGLU
8   PROALATHRSERTYRMETLYSALATYRTYR
9   ALALYSPHEGLYASNLEUASPALAALALYS
10   ARGASPPROASPVALGLNPROPROVALLEU
11   ASPPROARGARGALATHRTYRVALARGGLU
12   ALATHRTHRASPGLNASNGLYARGPHEASP
13   PHEASPHISILEPROASNGLYTHRTYRTYR
14   ILESERSERGLULEUTHRTRPSERALAGLN
15   SERASPGLYLYSTHRILETHRGLUGLYGLY
16   THRVALTHRLYSLEUVALTHRVALSERGLY
17   SERGLNPROGLNLYSVALLEULEUTHRARG

Samples:

sample_1: PA1324 (21-170), [U-100% C13; U-100% N15], 1 mM; NaCl 300 mM; BisTrisPropane 20 mM; D2O 10%; H2O 90%

sample_2: PA1324 (21-170), [U-10% C13; U-100% N15], 1 mM; NaCl 300 mM; BisTrisPropane 20 mM; D2O 10%; H2O 90%

sample_conditions: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
unknownnot availableisotropicsample_conditions

Software:

FELIX v98, Biosym/MSI/Accelrys - assignment, data processing, display

SPARKY, T.D. Goddard & D.G. Kneller, UCSF - assignment, spectral display

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 750 MHz
  • Varian Inova 800 MHz

Related Database Links:

PDB
DBJ BAK89069 BAP24320 BAP51904 GAA16866 GAJ52275
EMBL CAW28584 CCQ89061 CDH72000 CDH78337 CDI89494
GB AAG04713 AAT50168 ABJ10512 ABR82991 AEO76059
REF NP_250015 WP_003082734 WP_003112372 WP_003120489 WP_003123080

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks