BMRB Entry 6335

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6335
MolProbity Validation Chart

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Title:
Near complete chemical shift assignments for the zinc-bound redox switch domain of the E. coli Hsp33
Deposition date:
2004-10-04
Original release date:
2004-10-12
Authors:
Won, Hyung-Sik; Low, Lieh; Guzman, Roberto; Martinez-Yamout, Maria; Jakob, Ursula; Dyson, H.
Citation:

Citation: Won, Hyung-Sik; Low, Lieh; De Guzman, Roberto; Martinez-Yamout, Maria; Jakob, Ursula; Dyson, H.. "The zinc-dependent redox switch domain of the chaperone Hsp33 has a novel fold"  J. Mol. Biol. 341, 893-899 (2004).
PubMed: 15328602

Assembly members:

Assembly members:
E. coli heat shock protein 33 redox-switch domain, polymer, 62 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E. coli heat shock protein 33 redox-switch domain: MDVEFKCTCSRERCADALKT LPDEEVDSILAEDGEIDMHC DYCGNHYLFNAMDIAEIRNN AS

Data sets:
Data typeCount
1H chemical shifts392
13C chemical shifts242
15N chemical shifts63

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Hsp33-RSD1
2ZINC (II) ION2

Entities:

Entity 1, Hsp33-RSD 62 residues - Formula weight is not available

1   METASPVALGLUPHELYSCYSTHRCYSSER
2   ARGGLUARGCYSALAASPALALEULYSTHR
3   LEUPROASPGLUGLUVALASPSERILELEU
4   ALAGLUASPGLYGLUILEASPMETHISCYS
5   ASPTYRCYSGLYASNHISTYRLEUPHEASN
6   ALAMETASPILEALAGLUILEARGASNASN
7   ALASER

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: E. coli heat shock protein 33 redox-switch domain, [U-99% 13C; U-99% 15N], 1.0 – 2.0 mM

sample_2: E. coli heat shock protein 33 redox-switch domain, [U-99% 15N], 1.0 – 2.0 mM

Ex-cond_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 601 MHz

Related Database Links:

SWISS-PROT Q31VN3 Q0SZR7 P0A6Y7 P0A6Y6 P0A6Y5
REF NP_709173 NP_417860 NP_312270 NP_289940 AP_004389
PIR A86005
GenBank AAN82610 AAN44880 AAG58501 AAC76426 AAA58198
DBJ BAE77890 BAB37666
PDB

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