BMRB Entry 6328

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PDB ID: 1wo4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6328
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assessment of the robustness of a serendipitous zinc-binding fold: mutagenesis and protein grafting

Deposition date:

2004-09-24

Original release date:

2005-03-16

Authors:

Sharpe, B.; Liew, C.; Wilce, J.; Crossley, M.; Matthews, J.; Mackay, J.

Citation:

Citation: Sharpe, B.; Liew, C.; Kwan, A.; Wilce, J.; Crossley, M.; Matthews, J.; Mackay, J.. "Assessment of the robustness of a serendipitous zinc-binding fold: mutagenesis and protein grafting" Structure 13, 257-266 (2005).
PubMed: 15698569

Assembly members:

Assembly members:
CREB Binding Protein (E.C.2.3.1.48), polymer, 25 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemically synthesized

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CREB Binding Protein (E.C.2.3.1.48): AVSACALSKCAAAANVAAHM THCAK

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	134

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CREB Binding Protein	1
2	ZINC (II) ION	2

Entities:

Entity 1, CREB Binding Protein 25 residues - Formula weight is not available

1

ALA

VAL

SER

ALA

CYS

ALA

LEU

SER

LYS

CYS

2

ALA

ASN

VAL

ALA

HIS

MET

3

THR

HIS

CYS

ALA

LYS

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: CREB Binding Protein (E.C.2.3.1.48) 0.4 mM; TCEP 0.5 mM; ZnSO4 1 mM; D2O 100%

sample_cond_1: pH: 6.9; pressure: 1 atm; temperature: 275 K

Experiments:

Name	Sample	Sample state	Sample conditions
DQF-COSY	sample_1	not available	sample_cond_1
2D TOCSY	sample_1	not available	sample_cond_1
2D NOESY	sample_1	not available	sample_cond_1
E-COSY	sample_1	not available	sample_cond_1

Software:

xwinnmr v2.5 - processing

XEASY v1.3.13 - data analysis

DYANA v1.5 - data analysis

CNS v1.0 - refinement

NMR spectrometers:

Bruker DRX 600 MHz