BMRB Entry 6324

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6324
MolProbity Validation Chart

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Title:
Solution structure of the hypothetical protein Tm0979 from Thermotoga maritima
Deposition date:
2004-09-24
Original release date:
2004-12-22
Authors:
Gaspar, J.; Liu, C.; Vassall, K.; Stathopulos, P.; Meglei, G.; Stephen, R.; Pineda-Lucena, A.; Wu, B.; Yee, A.; Arrowsmith, C.; Meiering, E.
Citation:

Citation: Gaspar, J.; Liu, C.; Vassall, K.; Meglei, G.; Stephen, R.; Stathopulos, P.; Pineda-Lucena, A.; Wu, B.; Yee, A.; Arrowsmith, C.; Meiering, E.. "A novel member of the YchN-like fold: solution structure of the hypothetical protein Tm0979 from Thermotoga maritima "  Protein Sci. 14, 216-223 (2005).
PubMed: 15608123

Assembly members:

Assembly members:
hypothetical protein TM0979, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: not available   Kingdom: Bacteria   Genus/species: Thermotoga maritima

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hypothetical protein TM0979: MGSSHHHHHHSSGLVPRGSH MALVLVKYGTDHPVEKLKIR SAKAEDKIVLIQNGVFWALE ELETPAKVYAIKDDFLARGY SEEDSKVPLITYSEFIDLLE GEEKFIG

Data sets:
Data typeCount
1H chemical shifts594
13C chemical shifts281
15N chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VPR protein chain A1
2VPR protein chain B1

Entities:

Entity 1, VPR protein chain A 107 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METALALEUVALLEUVALLYSTYRGLYTHR
4   ASPHISPROVALGLULYSLEULYSILEARG
5   SERALALYSALAGLUASPLYSILEVALLEU
6   ILEGLNASNGLYVALPHETRPALALEUGLU
7   GLULEUGLUTHRPROALALYSVALTYRALA
8   ILELYSASPASPPHELEUALAARGGLYTYR
9   SERGLUGLUASPSERLYSVALPROLEUILE
10   THRTYRSERGLUPHEILEASPLEULEUGLU
11   GLYGLUGLULYSPHEILEGLY

Samples:

sample_1: hypothetical protein TM0979, [U-15N; U-13C], 1 mM; sodium phosphate 25 mM; NaCl 450 mM; sodium azide 0.01%; D2O 5%

sample_cond_1: pH: 6.5 na; temperature: 300 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
13C CT HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
HNHAnot availablenot availablenot available
CC(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HCC-TOCSYnot availablenot availablenot available
3D 15N and 13C TS H-CN-H NOESY-HSQCnot availablenot availablenot available
isotope-filtered 3D 13C HMQC-NOESY in D2Onot availablenot availablenot available

Software:

DYANA - structure solution

CNS - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 6010
PDB
GB AAD36058 AAZ04321 ABQ47763 ACB10146 ACM23775
REF NP_228787 WP_004080586 WP_011944167 YP_001245339 YP_001739829

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks