BMRB Entry 6322

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6322
MolProbity Validation Chart

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Title:
Chemical Shift assignment of the blue shifted inetrmediate state of Delta25-PYP
Deposition date:
2004-09-23
Original release date:
2005-07-25
Authors:
Bernard, Cedric; Houben, Klaartje; Derix, Nocky; Marks, David; van der Horst, Michael; Hellingwerf, Klaas; Boelens, Rolf; Kaptein, Robert; van Nuland, Nico
Citation:

Citation: Bernard, Cedric; Houben, Klaartje; Derix, Nocky; Marks, David; van der Horst, Michael; Hellingwerf, Klaas; Boelens, Rolf; Kaptein, Robert; van Nuland, Nico. "The solution structure of a transient photoreceptor intermediate: delta25 photoactive yellow protein."  Structure (Cambridge, MA, U. S.) 13, 953-962 (2005).
PubMed: 16004868

Assembly members:

Assembly members:
Delta25-PYP light state, polymer, 100 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Ectothiorhodospira halophila   Taxonomy ID: 1053   Superkingdom: Bacteria   Kingdom: Protista   Genus/species: Ectothiorhodospira halophila

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Delta25-PYP light state: LAFGAIQLDGDGNILQYNAA EGDITGRDPKQVIGKNFFKD VAPCTDSPEFYGKFKEGVAS GNLNTMFEYTFDYQMTPTKV KVHMKKALSGDSYWVFVKRV

Data sets:
Data typeCount
1H chemical shifts492
13C chemical shifts180
15N chemical shifts99

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Delta25-PYP light state1

Entities:

Entity 1, Delta25-PYP light state 100 residues - Formula weight is not available

1   LEUALAPHEGLYALAILEGLNLEUASPGLY
2   ASPGLYASNILELEUGLNTYRASNALAALA
3   GLUGLYASPILETHRGLYARGASPPROLYS
4   GLNVALILEGLYLYSASNPHEPHELYSASP
5   VALALAPROCYSTHRASPSERPROGLUPHE
6   TYRGLYLYSPHELYSGLUGLYVALALASER
7   GLYASNLEUASNTHRMETPHEGLUTYRTHR
8   PHEASPTYRGLNMETTHRPROTHRLYSVAL
9   LYSVALHISMETLYSLYSALALEUSERGLY
10   ASPSERTYRTRPVALPHEVALLYSARGVAL

Samples:

sample_1: Delta25-PYP light state, [U-13C; U-15N], 1 mM; phosphate buffer 50 mM

sample_2: Delta25-PYP light state, [U-15N], 1 mM; phosphate buffer 50 mM

sample_conditions: pH: 7; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCnot availablenot availablesample_conditions
1H 2D NOESYnot availablenot availablesample_conditions
1H 2D TOCSYnot availablenot availablesample_conditions
1H 15N NOESY HSQCnot availablenot availablesample_conditions
1H 15N TOCSY HSQCnot availablenot availablesample_conditions
HNCAnot availablenot availablesample_conditions
CBCA(CO)NHnot availablenot availablesample_conditions
HNCACBnot availablenot availablesample_conditions
13C Filtered 2D NOESYnot availablenot availablesample_conditions

Software:

NMRview v5.0.4 -

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 750 MHz
  • Bruker Avance 500 MHz

Related Database Links:

BMRB 18122 6321
PDB
EMBL CAA67391
GB AAA61735 AAB28014
SP P16113
AlphaFold P16113

Download HSQC peak lists in one of the following formats:
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