BMRB Entry 6314

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6314

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Title:
NMR assignment for cold-shock protein ribosome-binding factor A (RbfA) from Thermotoga maritima
Deposition date:
2004-09-07
Original release date:
2005-02-08
Authors:
Grimm, S.; Wohnert, Jens
Citation:

Citation: Grimm, S.; Wohnert, Jens. "Letter to the Editor: NMR assignments of the cold-shock protein ribosome-binding factor A (RbfA) from Thermotoga maritima"  J. Biomol. NMR 31, 73-74 (2005).

Assembly members:

Assembly members:
Ribosome binding factor A, polymer, 120 residues, 14133 Da.

Natural source:

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pUC19

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ribosome binding factor A: MNPAYRKAMLESEIQKLLME ALQQLRDPRLKKDFVTFSRV ELSKDKRYADVYVSFLGTPE ERKETVEILNRAKGFFRTFI AKNLRLYVAPEIRFYEDKGI EASVKVHQLLVQLGYDPLKD

Data sets:
Data typeCount
1H chemical shifts642
13C chemical shifts380
15N chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RbfA1

Entities:

Entity 1, RbfA 120 residues - 14133 Da.

1   METASNPROALATYRARGLYSALAMETLEU
2   GLUSERGLUILEGLNLYSLEULEUMETGLU
3   ALALEUGLNGLNLEUARGASPPROARGLEU
4   LYSLYSASPPHEVALTHRPHESERARGVAL
5   GLULEUSERLYSASPLYSARGTYRALAASP
6   VALTYRVALSERPHELEUGLYTHRPROGLU
7   GLUARGLYSGLUTHRVALGLUILELEUASN
8   ARGALALYSGLYPHEPHEARGTHRPHEILE
9   ALALYSASNLEUARGLEUTYRVALALAPRO
10   GLUILEARGPHETYRGLUASPLYSGLYILE
11   GLUALASERVALLYSVALHISGLNLEULEU
12   VALGLNLEUGLYTYRASPPROLEULYSASP

Samples:

sample_1: Ribosome binding factor A 1.0 mM

Ex-cond_1: pH: 4.5 na; temperature: 316 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACOnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
CC(CO)NHnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • BRUKER DRX 600 MHz
  • BRUKER DRX 800 MHz
  • BRUKER DRX 700 MHz

Related Database Links:

PDB
GB AAD35937 ABQ46101 ACB08434 ADA66180 AGL49782
REF NP_228664 WP_004080768 WP_011942775 WP_012310302 WP_038053978
SP A5IIS8 B1LCH6 Q9WZV9
AlphaFold A5IIS8 B1LCH6 Q9WZV9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks