BMRB Entry 6308
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6308
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Citation: Silvennoinen, Laura; Koivunen, Peppi; Myllyharju, Johanna; Kilpelainen, Ilkka; Permi, Perttu. "NMR Assignment of the N-terminal Domain a of the Glycoprotein Chaperone ERp57" J. Biomol. NMR 33, 136-136 (2005).
Assembly members:
ERp57, polymer, 113 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21(+)
Entity Sequences (FASTA):
ERp57: SDVLELTDDNFESRISDTGS
AGLMLVEFFAPWCGHCKRLA
PEYEAAATRLKGIVPLAKVD
CTANTNTCNKYGVSGYPTLK
IFRDGEEAGAYDGPRTADGI
VSHLKKQAGPASV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 339 |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 700 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ERp57 domain a | 1 |
Entities:
Entity 1, ERp57 domain a 113 residues - Formula weight is not available
| 1 | SER | ASP | VAL | LEU | GLU | LEU | THR | ASP | ASP | ASN | ||||
| 2 | PHE | GLU | SER | ARG | ILE | SER | ASP | THR | GLY | SER | ||||
| 3 | ALA | GLY | LEU | MET | LEU | VAL | GLU | PHE | PHE | ALA | ||||
| 4 | PRO | TRP | CYS | GLY | HIS | CYS | LYS | ARG | LEU | ALA | ||||
| 5 | PRO | GLU | TYR | GLU | ALA | ALA | ALA | THR | ARG | LEU | ||||
| 6 | LYS | GLY | ILE | VAL | PRO | LEU | ALA | LYS | VAL | ASP | ||||
| 7 | CYS | THR | ALA | ASN | THR | ASN | THR | CYS | ASN | LYS | ||||
| 8 | TYR | GLY | VAL | SER | GLY | TYR | PRO | THR | LEU | LYS | ||||
| 9 | ILE | PHE | ARG | ASP | GLY | GLU | GLU | ALA | GLY | ALA | ||||
| 10 | TYR | ASP | GLY | PRO | ARG | THR | ALA | ASP | GLY | ILE | ||||
| 11 | VAL | SER | HIS | LEU | LYS | LYS | GLN | ALA | GLY | PRO | ||||
| 12 | ALA | SER | VAL |
Samples:
sample_1: ERp57, [U-13C; U-15N], 0.2 – 1.5 mM
Cond_1: pH: 6.3; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC | not available | not available | not available |
| 13C CT-HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| CBCACONH | not available | not available | not available |
| 1H-15N NOESY HSQC | not available | not available | not available |
| CC(CO)NH | not available | not available | not available |
| HCC(CO)NH | not available | not available | not available |
| HNCO | not available | not available | not available |
Software:
VNMR v6.1 - spectra processing
SPARKY - peak picking
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks