BMRB Entry 6308

Title:
Structure of the domain a of the glycoprotein chaperone ERp57
Deposition date:
2004-08-30
Original release date:
2005-02-28
Authors:
Silvennoinen, Laura
Citation:

Citation: Silvennoinen, Laura; Koivunen, Peppi; Myllyharju, Johanna; Kilpelainen, Ilkka; Permi, Perttu. "NMR Assignment of the N-terminal Domain a of the Glycoprotein Chaperone ERp57"  J. Biomol. NMR 33, 136-136 (2005).

Assembly members:

Assembly members:
ERp57, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21(+)

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts339
15N chemical shifts111
1H chemical shifts700

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ERp57 domain a1

Entities:

Entity 1, ERp57 domain a 113 residues - Formula weight is not available

1   SERASPVALLEUGLULEUTHRASPASPASN
2   PHEGLUSERARGILESERASPTHRGLYSER
3   ALAGLYLEUMETLEUVALGLUPHEPHEALA
4   PROTRPCYSGLYHISCYSLYSARGLEUALA
5   PROGLUTYRGLUALAALAALATHRARGLEU
6   LYSGLYILEVALPROLEUALALYSVALASP
7   CYSTHRALAASNTHRASNTHRCYSASNLYS
8   TYRGLYVALSERGLYTYRPROTHRLEULYS
9   ILEPHEARGASPGLYGLUGLUALAGLYALA
10   TYRASPGLYPROARGTHRALAASPGLYILE
11   VALSERHISLEULYSLYSGLNALAGLYPRO
12   ALASERVAL

Samples:

sample_1: ERp57, [U-13C; U-15N], 0.2 – 1.5 mM

Cond_1: pH: 6.3; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
13C CT-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
1H-15N NOESY HSQCnot availablenot availablenot available
CC(CO)NHnot availablenot availablenot available
HCC(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available

Software:

VNMR v6.1 - spectra processing

SPARKY - peak picking

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks