BMRB Entry 6304

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6304

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Title:
Amide chemical shifts of mature human SUMO-1
Deposition date:
2004-08-27
Original release date:
2004-11-29
Authors:
McIntosh, Lawrence
Citation:

Citation: Macauley, Matthew; Errington, Wesley; Okon, Mark; Scharpf, Manuela; Mackereth, Cameron; Schulman, Brenda; McIntosh, Lawrence. "Structural and Dynamic Independence of Isopeptide-linked RanGAP1 and SUMO-1"  J. Biol. Chem. 279, 49131-49137 (2004).
PubMed: 15355965

Assembly members:

Assembly members:
SUMO-1, polymer, 100 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Homo sapian   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Animalia   Genus/species: Homo sapians

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SUMO-1: GSHMSDQEAKPSTEDLGDKK EGEYIKLKVIGQDSSEIHFK VKMTTHLKKLKESYCQRQGV PMNSLRFLFEGQRIADNHTP KELGMEEEDVIEVYQEQTGG

Data sets:
Data typeCount
1H chemical shifts93
15N chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SUMO-11

Entities:

Entity 1, SUMO-1 100 residues - Formula weight is not available

1   GLYSERHISMETSERASPGLNGLUALALYS
2   PROSERTHRGLUASPLEUGLYASPLYSLYS
3   GLUGLYGLUTYRILELYSLEULYSVALILE
4   GLYGLNASPSERSERGLUILEHISPHELYS
5   VALLYSMETTHRTHRHISLEULYSLYSLEU
6   LYSGLUSERTYRCYSGLNARGGLNGLYVAL
7   PROMETASNSERLEUARGPHELEUPHEGLU
8   GLYGLNARGILEALAASPASNHISTHRPRO
9   LYSGLULEUGLYMETGLUGLUGLUASPVAL
10   ILEGLUVALTYRGLNGLUGLNTHRGLYGLY

Samples:

sample: SUMO-1; KCl 100 mM; KPhos 10 mM; DTT 2 mM; D2O 10%

sample_conditions: pH: 6.5; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsamplenot availablesample_conditions
15N-editted NOESY-HSQCsamplenot availablesample_conditions
15N-editted TOCSY-HSQCsamplenot availablesample_conditions

Software:

NMRpipe -

Felix -

Sparky -

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

BMRB 16611 17536 25299
PDB
DBJ BAB22172 BAB27379 BAB93477 BAC40739 BAE35024
EMBL CAA67898 CAG31129 CAG46944 CAG46953 CAH92616
GB AAB39999 AAB40388 AAB40390 AAC39959 AAC50733
REF NP_001005781 NP_001005782 NP_001009672 NP_001030535 NP_001106146
SP A7WLH8 P63165 P63166 Q2EF74 Q5E9D1
TPG DAA32560
AlphaFold A7WLH8 P63165 P63166 Q2EF74 Q5E9D1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks