BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6298

Title: NMR STRUCTURE OF HUMAN HISTONE CHAPERONE, ASF1A

Deposition date: 2004-08-26 Original release date: 2005-11-07

Authors: MOUSSON, F.; LAUTRETTE, A.; THURET, J.; AGEZ, M.; AMIGUES, B.; BECKER, E.; COURBEYRETTE, R.; NEUMANN, J.; GUEROIS, R.; MANN, C.; OCHSENBEIN, F.

Citation: Mousson, Florence; Couprie, Joel; Thuret, Jean-Yves; Neumann, Jean-Michel; Mann, Carl; Ochsenbein, Francoise. "Letter to the Editor: 1H, 13C and 15N Resonance Assignments of the Conserved Core of hAsf1 A"  J. Biomol. NMR 29, 413-414 (2004).

Assembly members:
ASF1 anti-silencing function 1 homolog A, polymer, 156 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PETM30

Entity Sequences (FASTA):
ASF1 anti-silencing function 1 homolog A: MAKVQVNNVVVLDNPSPFYN PFQFEITFECIEDLSEDLEW KIIYVGSAESEEYDQVLDSV LVGPVPAGRHMFVFQADAPN PGLIPDADAVGVTVVLITCT YRGQEFIRVGYYVNNEYTET ELRENPPVKPDFSKLQRNIL ASNPRVTRFHINWEDN

Data sets:
Data typeCount
1H chemical shifts1091
13C chemical shifts668
15N chemical shifts166

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Four-helix bundle model1

Entities:

Entity 1, Four-helix bundle model 156 residues - Formula weight is not available

1   METALALYSVALGLNVALASNASNVALVAL
2   VALLEUASPASNPROSERPROPHETYRASN
3   PROPHEGLNPHEGLUILETHRPHEGLUCYS
4   ILEGLUASPLEUSERGLUASPLEUGLUTRP
5   LYSILEILETYRVALGLYSERALAGLUSER
6   GLUGLUTYRASPGLNVALLEUASPSERVAL
7   LEUVALGLYPROVALPROALAGLYARGHIS
8   METPHEVALPHEGLNALAASPALAPROASN
9   PROGLYLEUILEPROASPALAASPALAVAL
10   GLYVALTHRVALVALLEUILETHRCYSTHR
11   TYRARGGLYGLNGLUPHEILEARGVALGLY
12   TYRTYRVALASNASNGLUTYRTHRGLUTHR
13   GLULEUARGGLUASNPROPROVALLYSPRO
14   ASPPHESERLYSLEUGLNARGASNILELEU
15   ALASERASNPROARGVALTHRARGPHEHIS
16   ILEASNTRPGLUASPASN

Samples:

sample_1: ASF1 anti-silencing function 1 homolog A, [U-15N; U-13C], 1 mM; tris-D11 20 mM; EDTA 1 mM; DSS 0.1 mM; NaN3 0.1 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 7.4; temperature: 298 K; ionic strength: 20 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablesample_cond_1
3D 15N-separated NOESYsample_1not availablesample_cond_1
HNHAsample_1not availablesample_cond_1

Software:

XWINNMR v3.1 - collection, processing

SPARKY v3.106 - data analysis

ARIA v1.1 - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAA96542 BAB15228 BAB25269 BAB26770 BAB28198
EMBL CAB43363 CAG33628
GB AAD34093 AAF29110 AAH10878 AAH27628 AAI12652
REF NP_001038155 NP_001069961 NP_001086449 NP_001099859 NP_001159660
SP Q2KIG1 Q3C1E9 Q69DB9 Q9CQE6 Q9Y294
TPG DAA26326
AlphaFold Q9CQE6

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts