BMRB Entry 6292

Title:
1H, 15N and 13C sidechain and backbone assignments of the alkaline proteinase inhibitor APRin from Pseudomonas aeruginosa
Deposition date:
2004-08-20
Original release date:
2008-07-01
Authors:
Arumugam, Sengodagounder; Gray, Robert; Lane, Andrew
Citation:

Citation: Arumugam, Sengodagounder; Gray, Robert; Lane, Andrew. "Letter to the Editor: 1H, 15N and 13C assignments of the alkaline proteinase inhibitor APRin from Pseudomonas aeruginosa "  J. Biomol. NMR 31, 265-266 (2005).
PubMed: 15803404

Assembly members:

Assembly members:
Alkaline protease inhibitor, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b+

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts613
13C chemical shifts410
15N chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1APRin1

Entities:

Entity 1, APRin 106 residues - Formula weight is not available

1   SERSERLEUILELEULEUSERALASERASP
2   LEUALAGLYGLNTRPTHRLEUGLNGLNASP
3   GLUALAPROALAILECYSHISLEUGLULEU
4   ARGASPSERGLUVALALAGLUALASERGLY
5   TYRASPLEUGLYGLYASPTHRALACYSLEU
6   THRARGTRPLEUPROSERGLUPROARGALA
7   TRPARGPROTHRPROALAGLYILEALALEU
8   LEUGLUARGGLYGLYLEUTHRLEUMETLEU
9   LEUGLYARGGLNGLYGLUGLYASPTYRARG
10   VALGLNLYSGLYASPGLYGLYGLNLEUVAL
11   LEUARGARGALATHRPRO

Samples:

sample_1: Alkaline protease inhibitor, [U-90% 15N], 0.8 mM; sodium phosphate 20 mM; potassium chloride 100 mM

sample_2: Alkaline protease inhibitor, [U-95% 13C; U-90% 15N], 1.7 mM; sodium phosphate 20 mM; potassium chloride 100 mM

Condition_1: pH: 6.5; temperature: 298 K; ionic strength: 0.12 M

Experiments:

NameSampleSample stateSample conditions
15N/1H 2D HSQCnot availablenot availablenot available
3D TOCSY-HSQC (15N/1H)not availablenot availablenot available
3D NOESY-HSQC (15N/1H)not availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HCACOnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

NMRPipe v2.3 Rev 2004.148.12.51 - Data processing

Sparky v3.106 - Data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAK88991 BAP20448 BAP52029 GAA16794 GAJ52350
EMBL CAA45859 CAW28816 CCQ85472 CDH72198 CDH78463
GB AAG04639 AAT49619 ABJ10438 AEO76262 AFM66153
REF NP_249941 WP_003082543 WP_003086733 WP_003114968 WP_003116378
SP Q03026
AlphaFold Q03026

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks