BMRB Entry 6292
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6292
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Citation: Arumugam, Sengodagounder; Gray, Robert; Lane, Andrew. "Letter to the Editor: 1H, 15N and 13C assignments of the alkaline proteinase
inhibitor APRin from Pseudomonas aeruginosa " J. Biomol. NMR 31, 265-266 (2005).
PubMed: 15803404
Assembly members:
Alkaline protease inhibitor, polymer, 106 residues, Formula weight is not available
Natural source: Common Name: pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Eubacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b+
Entity Sequences (FASTA):
Alkaline protease inhibitor: SSLILLSASDLAGQWTLQQD
EAPAICHLELRDSEVAEASG
YDLGGDTACLTRWLPSEPRA
WRPTPAGIALLERGGLTLML
LGRQGEGDYRVQKGDGGQLV
LRRATP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 613 |
| 13C chemical shifts | 410 |
| 15N chemical shifts | 100 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | APRin | 1 |
Entities:
Entity 1, APRin 106 residues - Formula weight is not available
| 1 | SER | SER | LEU | ILE | LEU | LEU | SER | ALA | SER | ASP | ||||
| 2 | LEU | ALA | GLY | GLN | TRP | THR | LEU | GLN | GLN | ASP | ||||
| 3 | GLU | ALA | PRO | ALA | ILE | CYS | HIS | LEU | GLU | LEU | ||||
| 4 | ARG | ASP | SER | GLU | VAL | ALA | GLU | ALA | SER | GLY | ||||
| 5 | TYR | ASP | LEU | GLY | GLY | ASP | THR | ALA | CYS | LEU | ||||
| 6 | THR | ARG | TRP | LEU | PRO | SER | GLU | PRO | ARG | ALA | ||||
| 7 | TRP | ARG | PRO | THR | PRO | ALA | GLY | ILE | ALA | LEU | ||||
| 8 | LEU | GLU | ARG | GLY | GLY | LEU | THR | LEU | MET | LEU | ||||
| 9 | LEU | GLY | ARG | GLN | GLY | GLU | GLY | ASP | TYR | ARG | ||||
| 10 | VAL | GLN | LYS | GLY | ASP | GLY | GLY | GLN | LEU | VAL | ||||
| 11 | LEU | ARG | ARG | ALA | THR | PRO |
Samples:
sample_1: Alkaline protease inhibitor, [U-90% 15N], 0.8 mM; sodium phosphate 20 mM; potassium chloride 100 mM
sample_2: Alkaline protease inhibitor, [U-95% 13C; U-90% 15N], 1.7 mM; sodium phosphate 20 mM; potassium chloride 100 mM
Condition_1: pH: 6.5; temperature: 298 K; ionic strength: 0.12 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N/1H 2D HSQC | not available | not available | not available |
| 3D TOCSY-HSQC (15N/1H) | not available | not available | not available |
| 3D NOESY-HSQC (15N/1H) | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HCACO | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| C(CO)NH | not available | not available | not available |
| HNHA | not available | not available | not available |
Software:
NMRPipe v2.3 Rev 2004.148.12.51 - Data processing
Sparky v3.106 - Data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAK88991 BAP20448 BAP52029 GAA16794 GAJ52350 |
| EMBL | CAA45859 CAW28816 CCQ85472 CDH72198 CDH78463 |
| GB | AAG04639 AAT49619 ABJ10438 AEO76262 AFM66153 |
| REF | NP_249941 WP_003082543 WP_003086733 WP_003114968 WP_003116378 |
| SP | Q03026 |
| AlphaFold | Q03026 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks