BMRB Entry 6291
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6291
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Wu, Bin; Yee, Adelina; Huang, Yuanpeng; Ramelot, Theresa; Lemak, Alexander; Kennedy, Michael; Arrowsmith, Cheryl. "Solution structure of hypothetical membrane protein ta0354 from Thermoplasma
acidophilum" The BMRB entry is the only known published source for the data. (2005).
Assembly members:
hypothetical membrane protein ta0354, polymer, 53 residues, 6193 Da.
Natural source: Common Name: Thermoplasma acidophilum Taxonomy ID: 2303 Superkingdom: Archaea Kingdom: not available Genus/species: thermoplasma acidophilum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET15B
Entity Sequences (FASTA):
hypothetical membrane protein ta0354: RNLSDRAKFESMINSPSKSV
FVRNLNELEALAVRLGKSYR
IQLDQAKEKWKVK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 338 |
| 13C chemical shifts | 205 |
| 15N chemical shifts | 48 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ta0354_69_121 | 1 |
Entities:
Entity 1, ta0354_69_121 53 residues - 6193 Da.
| 1 | ARG | ASN | LEU | SER | ASP | ARG | ALA | LYS | PHE | GLU | ||||
| 2 | SER | MET | ILE | ASN | SER | PRO | SER | LYS | SER | VAL | ||||
| 3 | PHE | VAL | ARG | ASN | LEU | ASN | GLU | LEU | GLU | ALA | ||||
| 4 | LEU | ALA | VAL | ARG | LEU | GLY | LYS | SER | TYR | ARG | ||||
| 5 | ILE | GLN | LEU | ASP | GLN | ALA | LYS | GLU | LYS | TRP | ||||
| 6 | LYS | VAL | LYS |
Samples:
sample_1: hypothetical membrane protein ta0354, [U-13C; U-15N], 1 mM; NaCl 450 mM
cond_set1: pH: 6.5; temperature: 298 K; ionic strength: 0.45 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| CBCAcoNH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNcoCA | not available | not available | not available |
| CCTOCSY_NH | not available | not available | not available |
| HCCTOCSY_NH | not available | not available | not available |
| HNHA | not available | not available | not available |
| 13C HSQC | not available | not available | not available |
| HCCHCOSY | not available | not available | not available |
| HCCHTOCSY | not available | not available | not available |
| 15N NOESY | not available | not available | not available |
| 13C NOESY | not available | not available | not available |
| 13,13C 4d CCNOESY | not available | not available | not available |
Software:
SPARKY v3.95 - spectral analysis, assignemt
NMR spectrometers:
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks