BMRB Entry 6287
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6287
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Mackereth, Cameron; Schaerpf, Manuela; Gentile, Lisa; MacIntosh, Scott; Slupsky, Carolin; Gentile, Lisa; McIntosh, Lawrence. "Diversity in structure and function of the Ets family pointed domains" J. Mol. Biol. ., .-..
Assembly members:
GABPalpha pointed domain, polymer, 91 residues, 10320 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
GABPalpha pointed domain: GSHMAALEGYRKEQERLGIP
YDPIHWSTDQVLHWVVWVMK
EFSMTDIDLTTLNISGRELC
SLNQEDFFQRVPRGEILWSH
LELLRKYVLAS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 663 |
| 13C chemical shifts | 414 |
| 15N chemical shifts | 86 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GABPalpha pointed domain | 1 |
Entities:
Entity 1, GABPalpha pointed domain 91 residues - 10320 Da.
| 1 | GLY | SER | HIS | MET | ALA | ALA | LEU | GLU | GLY | TYR | ||||
| 2 | ARG | LYS | GLU | GLN | GLU | ARG | LEU | GLY | ILE | PRO | ||||
| 3 | TYR | ASP | PRO | ILE | HIS | TRP | SER | THR | ASP | GLN | ||||
| 4 | VAL | LEU | HIS | TRP | VAL | VAL | TRP | VAL | MET | LYS | ||||
| 5 | GLU | PHE | SER | MET | THR | ASP | ILE | ASP | LEU | THR | ||||
| 6 | THR | LEU | ASN | ILE | SER | GLY | ARG | GLU | LEU | CYS | ||||
| 7 | SER | LEU | ASN | GLN | GLU | ASP | PHE | PHE | GLN | ARG | ||||
| 8 | VAL | PRO | ARG | GLY | GLU | ILE | LEU | TRP | SER | HIS | ||||
| 9 | LEU | GLU | LEU | LEU | ARG | LYS | TYR | VAL | LEU | ALA | ||||
| 10 | SER |
Samples:
sample_1: GABPalpha pointed domain, [U-99% 15N], 0.5 – 2.5 mM; NaP 20 mM; NaCl 20 mM
sample_2: GABPalpha pointed domain, [U-99% 13C; U-99% 15N], 1.5 mM; NaP 20 mM; NaCl 20 mM
sample_3: GABPalpha pointed domain, [U-10% 13C; U-99% 15N], 1.5 mM; NaP 20 mM; NaCl 20 mM
generell_exp_condition: pH: 7.2; temperature: 303 K; ionic strength: 0.4 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C HSQC | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HBCBCACONH | not available | not available | not available |
| HNCO | not available | not available | not available |
| H(CCO)NH-TOCSY | not available | not available | not available |
| C(CO)NH-TOCSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
Software:
FELIX v2000 - processing
NMRPipe-Draw - processing
SPARKY v3 - assignment
NMR spectrometers:
- Varian Inova 600 MHz
- Varian Unity 500 MHz
Related Database Links:
| BMRB | 5401 |
| PDB | |
| DBJ | BAA02575 BAD96884 BAE24181 BAE26442 BAE26687 |
| GB | AAA53030 AAA65706 AAH13562 AAH35031 AAH52448 |
| REF | NP_001068905 NP_001102311 NP_001184226 NP_001253514 NP_002031 |
| SP | Q00422 Q06546 |
| TPG | DAA33658 |
| AlphaFold | Q00422 Q06546 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks