BMRB Entry 6283
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6283
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Liepinsh, Edvards; Rakonjac, Marija; Boissonneault, Vincent; Provost, Patrick; Samuelsson, Bengt; Radmark, Olof; Otting, Gottfried. "Letter to the Editor: NMR Structure of Human Coactosin-Like Protein" J. Biomol. NMR 30, 353-356 (2004).
Assembly members:
coactosin like protein, polymer, 145 residues, 16269 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
coactosin like protein: GIRMATKIDKEACRAAYNLV
RDDGSAVIWVTFKYDGSTIV
PGEQGAEYQHFIQQCTDDVR
LFAFVRFTTGDAMSKRSKFA
LITWIGENVSGLQRAKTGTD
KTLVKEVVQNFAKEFVISDR
KELEEDFIKSELKKAGGANY
DAQTE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 996 |
| 15N chemical shifts | 154 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | coactosin like protein | 1 |
Entities:
Entity 1, coactosin like protein 145 residues - 16269 Da.
| 1 | GLY | ILE | ARG | MET | ALA | THR | LYS | ILE | ASP | LYS | ||||
| 2 | GLU | ALA | CYS | ARG | ALA | ALA | TYR | ASN | LEU | VAL | ||||
| 3 | ARG | ASP | ASP | GLY | SER | ALA | VAL | ILE | TRP | VAL | ||||
| 4 | THR | PHE | LYS | TYR | ASP | GLY | SER | THR | ILE | VAL | ||||
| 5 | PRO | GLY | GLU | GLN | GLY | ALA | GLU | TYR | GLN | HIS | ||||
| 6 | PHE | ILE | GLN | GLN | CYS | THR | ASP | ASP | VAL | ARG | ||||
| 7 | LEU | PHE | ALA | PHE | VAL | ARG | PHE | THR | THR | GLY | ||||
| 8 | ASP | ALA | MET | SER | LYS | ARG | SER | LYS | PHE | ALA | ||||
| 9 | LEU | ILE | THR | TRP | ILE | GLY | GLU | ASN | VAL | SER | ||||
| 10 | GLY | LEU | GLN | ARG | ALA | LYS | THR | GLY | THR | ASP | ||||
| 11 | LYS | THR | LEU | VAL | LYS | GLU | VAL | VAL | GLN | ASN | ||||
| 12 | PHE | ALA | LYS | GLU | PHE | VAL | ILE | SER | ASP | ARG | ||||
| 13 | LYS | GLU | LEU | GLU | GLU | ASP | PHE | ILE | LYS | SER | ||||
| 14 | GLU | LEU | LYS | LYS | ALA | GLY | GLY | ALA | ASN | TYR | ||||
| 15 | ASP | ALA | GLN | THR | GLU |
Samples:
sample: coactosin like protein, [U-90% 15N], 0.15 mM; Tris buffer 8 mM; DTT 1 mM; NaCl 50 mM; D2O 5%
sample_conditions: temperature: 298 K; pH: 7
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample | not available | sample_conditions |
| 3D_NOESY-HSQC | sample | not available | sample_conditions |
| 3D_TOCSY-HSQC | sample | not available | sample_conditions |
| 2D_NOESY | sample | not available | sample_conditions |
| 2D_TOCSY | sample | not available | sample_conditions |
| 2D_DQF_COSY | sample | not available | sample_conditions |
Software:
XWINNMR v2.8 - peak assignments
PROSA v2.8 - spectrum processing
DYANA v2.5 - structure calculation
OPAL v2.2 - structure refinement
NMR spectrometers:
- Bruker DMX 600 MHz
- Varian UNITY 800 MHz
Related Database Links:
| BMRB | 6071 |
| PDB | |
| DBJ | BAG38040 BAJ20994 |
| GB | AAA88022 AAH10039 AAH10884 AAH16702 AAH42970 |
| REF | NP_001040058 NP_001248084 NP_066972 XP_001499912 XP_002761262 |
| SP | Q14019 Q2HJ57 |
| TPG | DAA20312 |
| AlphaFold | Q14019 Q2HJ57 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks