BMRB Entry 6280

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6280

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Title:
Resonance assignment of the double-stranded RNA-binding domains of adenosine deaminase acting on RNA
Deposition date:
2004-07-30
Original release date:
2005-02-08
Authors:
Stefl, Richard; Skrisovska, Lenka; Xu, Ming; Emeson, Ronald; Allain, Frederic
Citation:

Citation: Stefl, Richard; Skrisovska, Lenka; Xu, Ming; Emeson, Ronald; Allain, Frederic. "Letter to the Editor: Resonance assignments of the double-stranded RNA-binding of adenosine deaminase acting on RNA 2 (ADAR2)"  J. Biomol. NMR 31, 71-72 (2005).

Assembly members:

Assembly members:
double-stranded RNA-binding domains of adenosine deaminase acting on RNA, polymer, 294 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
double-stranded RNA-binding domains of adenosine deaminase acting on RNA: MQYKLILNGKTLKGETTTEA VDAATAEKVFKQYANDNGVD GEWTYDDATKTFTVTEGSPG PVLPKNALMQLNEIKPGLQY MLLSQTGPVHAPLFVMSVEV NGQVFEGSGPTKKKAKLHAA EKALRSFVQFPNASEAHLAM GRTLSVNTDFTSDQADFPDT LFNGFETPDKSEPPFYVGSN GDDSFSSSGDVSLSASPVPA SLTQPPLPIPPPFPPPSGKN PVMILNELRPGLKYDFLSES GESHAKSFVMSVVVDGQFFE GSGRNKKLAKARAAQSALAT VFNLHLLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts613
15N chemical shifts222
1H chemical shifts1272

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dsRBD12 of ADAR21

Entities:

Entity 1, dsRBD12 of ADAR2 294 residues - Formula weight is not available

1   METGLNTYRLYSLEUILELEUASNGLYLYS
2   THRLEULYSGLYGLUTHRTHRTHRGLUALA
3   VALASPALAALATHRALAGLULYSVALPHE
4   LYSGLNTYRALAASNASPASNGLYVALASP
5   GLYGLUTRPTHRTYRASPASPALATHRLYS
6   THRPHETHRVALTHRGLUGLYSERPROGLY
7   PROVALLEUPROLYSASNALALEUMETGLN
8   LEUASNGLUILELYSPROGLYLEUGLNTYR
9   METLEULEUSERGLNTHRGLYPROVALHIS
10   ALAPROLEUPHEVALMETSERVALGLUVAL
11   ASNGLYGLNVALPHEGLUGLYSERGLYPRO
12   THRLYSLYSLYSALALYSLEUHISALAALA
13   GLULYSALALEUARGSERPHEVALGLNPHE
14   PROASNALASERGLUALAHISLEUALAMET
15   GLYARGTHRLEUSERVALASNTHRASPPHE
16   THRSERASPGLNALAASPPHEPROASPTHR
17   LEUPHEASNGLYPHEGLUTHRPROASPLYS
18   SERGLUPROPROPHETYRVALGLYSERASN
19   GLYASPASPSERPHESERSERSERGLYASP
20   VALSERLEUSERALASERPROVALPROALA
21   SERLEUTHRGLNPROPROLEUPROILEPRO
22   PROPROPHEPROPROPROSERGLYLYSASN
23   PROVALMETILELEUASNGLULEUARGPRO
24   GLYLEULYSTYRASPPHELEUSERGLUSER
25   GLYGLUSERHISALALYSSERPHEVALMET
26   SERVALVALVALASPGLYGLNPHEPHEGLU
27   GLYSERGLYARGASNLYSLYSLEUALALYS
28   ALAARGALAALAGLNSERALALEUALATHR
29   VALPHEASNLEUHISLEULEUGLUHISHIS
30   HISHISHISHIS

Samples:

sample_1: double-stranded RNA-binding domains of adenosine deaminase acting on RNA, [U-85% 2H; U-13C; U-15N], 0.8 mM

ex-cond_1: pH: 8.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
[1H,15N]-TROSYnot availablenot availablenot available
[1H,15N]-TROSY-HNCAnot availablenot availablenot available
[1H,15N]-TROSY-HNCACBnot availablenot availablenot available
3D NOESY-[1H,15N,1H]-TROSYnot availablenot availablenot available
[1H,15N]-HSQCnot availablenot availablenot available
[1H,13C]-HSQCnot availablenot availablenot available
TOCSYnot availablenot availablenot available
2D [1H,1H]-NOESYnot availablenot availablenot available
3D [1H,15N,1H]-NOESYnot availablenot availablenot available
3D [1H,13C,1H]-NOESYnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker DRX 750 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAE23623 BAE24831 BAE27830
GB AAA96755 AAB18134 AAI65982 AAK17102 AAL01301
PRF 2204394A
REF NP_001020008 NP_001104525 NP_001104526 NP_001104527 NP_037026
SP P51400 Q91ZS8
AlphaFold P51400 Q91ZS8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks