BMRB Entry 6278

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for yeast Sed5p
Published: 2004-09-28

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6278

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for yeast Sed5p

Deposition date:

2004-07-28

Original release date:

2004-09-28

Authors:

Chen, Xiaocheng; Rizo, Josep

Citation:

Citation: Yamaguchi, Tomohiro; Dulubova, Irina; Min, Sang-Won; Chen, Xiaocheng; Rizo, Josep; Sudhof, Thomas. "Sly1 Binds to Golgi and ER Syntaxins via a Conserved N-Terminal Peptide Motif" Dev. Cell 2, 295-305 (2002).
PubMed: 11879635

Assembly members:

Assembly members:
Sed5p, polymer, 127 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sed5p: KSVSEFQKKASGIAHEISST AQLLSKLAVLAKRKPMFNDN PVEIAELSFLIKRKIYAIEQ SLVQLSQLKKTDVNGNTSNQ SSKQPSAVQHSKNVVNLLNT QMKNISGSFKDVLEERQRLE MANKDRW

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	341
15N chemical shifts	113
1H chemical shifts	113

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Yeast Sed5p	1

Entities:

Entity 1, Yeast Sed5p 127 residues - Formula weight is not available

1

LYS

SER

VAL

SER

GLU

PHE

GLN

LYS

ALA

2

SER

GLY

ILE

ALA

HIS

GLU

ILE

SER

THR

3

ALA

GLN

LEU

SER

LYS

LEU

ALA

VAL

LEU

4

ALA

LYS

ARG

LYS

PRO

MET

PHE

ASN

ASP

ASN

5

PRO

VAL

GLU

ILE

ALA

GLU

LEU

SER

PHE

LEU

6

ILE

LYS

ARG

LYS

ILE

TYR

ALA

ILE

GLU

GLN

7

SER

LEU

VAL

GLN

LEU

SER

GLN

LEU

LYS

8

THR

ASP

VAL

ASN

GLY

ASN

THR

SER

ASN

GLN

9

SER

LYS

GLN

PRO

SER

ALA

VAL

GLN

HIS

10

SER

LYS

ASN

VAL

ASN

LEU

ASN

THR

11

GLN

MET

LYS

ASN

ILE

SER

GLY

SER

PHE

LYS

12

ASP

VAL

LEU

GLU

ARG

GLN

ARG

LEU

GLU

13

MET

ALA

ASN

LYS

ASP

ARG

TRP

Samples:

sample_1: Sed5p, [U-13C; U-15N], 0.5 mM

Ex-cond_1: pH: 6.5; temperature: 302 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCO	not available	not available	not available
1H-15N NOESY	not available	not available	not available
HNCACB	not available	not available	not available
CBCACONH	not available	not available	not available

Software:

NMRView v4.12 - analyze NMR data

NMR spectrometers:

Varian INOVA 600 MHz

DBJ	GAA24913
EMBL	CAA47390 CAA97549 CAY81264
GB	AHY78432 EDN59573 EDV09334 EDZ70730 EEU04509
REF	NP_013126
SP	Q01590
TPG	DAA09344
AlphaFold	Q01590