BMRB Entry 6277

Name: NMR solution structure of the ubiquitin conjugating enzyme UbcH5B
Published: 2005-02-21

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6277

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR solution structure of the ubiquitin conjugating enzyme UbcH5B

Deposition date:

2004-07-28

Original release date:

2005-02-21

Authors:

Houben, Klaartje; Dominguez, Cyril; van Schaik, Frederik; Timmers, Marc; Bonvin, Alexandre; Boelens, Rolf

Citation:

Citation: Houben, Klaartje; Dominguez, Cyril; van Schaik, Frederik; Timmers, Marc; Bonvin, Alexandre; Boelens, Rolf. "Solution structure of the ubiquitin-conjugating enzyme UbcH5B" J. Mol. Biol. 344, 513-526 (2004).
PubMed: 15522302

Assembly members:

Assembly members:
UbcH5B, polymer, 147 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UbcH5B: MALKRIHKELNDLARDPPAQ CSAGPVGDDMFHWQATIMGP NDSPYQGGVFFLTIHFPTDY PFKPPKVAFTTRIYHPNINS NGSICLDILRSQWSPALTIS KVLLSICSLLCDPNPDDPLV PEIARIYKTDREKYNRIARE WTQKYAM

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts

Data type	Count
13C chemical shifts	519
15N chemical shifts	144
1H chemical shifts	900

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UbcH5B	1

Entities:

Entity 1, UbcH5B 147 residues - Formula weight is not available

1

MET

ALA

LEU

LYS

ARG

ILE

HIS

LYS

GLU

LEU

2

ASN

ASP

LEU

ALA

ARG

ASP

PRO

ALA

GLN

3

CYS

SER

ALA

GLY

PRO

VAL

GLY

ASP

MET

4

PHE

HIS

TRP

GLN

ALA

THR

ILE

MET

GLY

PRO

5

ASN

ASP

SER

PRO

TYR

GLN

GLY

VAL

PHE

6

PHE

LEU

THR

ILE

HIS

PHE

PRO

THR

ASP

TYR

7

PRO

PHE

LYS

PRO

LYS

VAL

ALA

PHE

THR

8

THR

ARG

ILE

TYR

HIS

PRO

ASN

ILE

ASN

SER

9

ASN

GLY

SER

ILE

CYS

LEU

ASP

ILE

LEU

ARG

10

SER

GLN

TRP

SER

PRO

ALA

LEU

THR

ILE

SER

11

LYS

VAL

LEU

SER

ILE

CYS

SER

LEU

12

CYS

ASP

PRO

ASN

PRO

ASP

PRO

LEU

VAL

13

PRO

GLU

ILE

ALA

ARG

ILE

TYR

LYS

THR

ASP

14

ARG

GLU

LYS

TYR

ASN

ARG

ILE

ALA

ARG

GLU

15

TRP

THR

GLN

LYS

TYR

ALA

MET

Samples:

sample: UbcH5B, [U-15N; U-13C], 0.3 mM; KCl 150 mM; Kpi 20 mM; ZnCl2 0.01 mM

sample_conditions: ionic strength: 0.15 M; pH: 7; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC	sample	not available	sample_conditions
HNCACB	sample	not available	sample_conditions
HcCH-TOSCY	sample	not available	sample_conditions
hCCH-TOCSY	sample	not available	sample_conditions
1H15N_TOCSY	sample	not available	sample_conditions
1H15N_NOESY	sample	not available	sample_conditions

Software:

No software information available

NMR spectrometers:

unknown unknown 0 MHz

BMRB	4866
PDB	1UR6 1W4U 1X23 2CLW 2ESK 2ESO 2ESP 2ESQ 2FUH 3A33 3EB6 3JVZ 3JW0 3L1Y 3L1Z 3RPG 3TGD 3ZNI 4A49 4A4B 4A4C 4AUQ 4DDG 4DDI 4LDT 4R8P 4WZ3
DBJ	BAA87330 BAB23116 BAC28070 BAC33981 BAC36940
EMBL	CAG31534 CAH93209 CAJ81618 CDQ70075 CDQ72611
GB	AAA85100 AAA85101 AAA85102 AAA91460 AAA91461
PRF	2111484A
REF	NP_001016064 NP_001026324 NP_001032369 NP_001039961 NP_001072141
SP	P61077 P61078 P61079 P62837 P62838
TPG	DAA27344
AlphaFold	P61077 P61078 P61079 P62837 P62838