BMRB Entry 6272
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6272
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: AMEGBEY, GODWIN; CHANG, ZHAN; STOTHARD, PAUL; YEE, ADELINDA; ARROWSMITH, CHERYL; WISHART, DAVID. "Letter to the Editor: 1H, 13C AND 15N NMR Assignments of MTH0776 from
Methanobacterium thermoautotrophicum" J. Biomol. NMR 30, 459-460 (2004).
Assembly members:
MTH0776, polymer, 121 residues, 11397.37 Da.
Natural source: Common Name: Methanobacterium thermoautotrophicum Taxonomy ID: 145262 Superkingdom: Archaea Kingdom: not available Genus/species: Methanothermobacter thermautotrophicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
MTH0776: MGSSHHHHHHSSGLVPRGSH
MTFCLETYLQQSGEYEIHMK
RAGFRECAAMIEKKARRVVH
IKPGEKILGARIIGIPPVPI
GIDEERSTVMIPYTKPCYGT
AVVELPVDPEEIERILEVAE
P
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 829 |
| 13C chemical shifts | 463 |
| 15N chemical shifts | 110 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Conserved protein from methanobacterium thermoautotrophicum | 1 |
Entities:
Entity 1, Conserved protein from methanobacterium thermoautotrophicum 121 residues - 11397.37 Da.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | THR | PHE | CYS | LEU | GLU | THR | TYR | LEU | GLN | ||||
| 4 | GLN | SER | GLY | GLU | TYR | GLU | ILE | HIS | MET | LYS | ||||
| 5 | ARG | ALA | GLY | PHE | ARG | GLU | CYS | ALA | ALA | MET | ||||
| 6 | ILE | GLU | LYS | LYS | ALA | ARG | ARG | VAL | VAL | HIS | ||||
| 7 | ILE | LYS | PRO | GLY | GLU | LYS | ILE | LEU | GLY | ALA | ||||
| 8 | ARG | ILE | ILE | GLY | ILE | PRO | PRO | VAL | PRO | ILE | ||||
| 9 | GLY | ILE | ASP | GLU | GLU | ARG | SER | THR | VAL | MET | ||||
| 10 | ILE | PRO | TYR | THR | LYS | PRO | CYS | TYR | GLY | THR | ||||
| 11 | ALA | VAL | VAL | GLU | LEU | PRO | VAL | ASP | PRO | GLU | ||||
| 12 | GLU | ILE | GLU | ARG | ILE | LEU | GLU | VAL | ALA | GLU | ||||
| 13 | PRO |
Samples:
sample: MTH0776, [U-100% 13C; U-100% 15N], 1 mM; NaH2PO4 50 mM; NaCL 300 mM; DTT 15 mM; NaN3 0.06%; DSS 0.1 mM
sample_conditions: pH: 6.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 15N HSQC | sample | not available | sample_conditions |
| 2D 13C HSQC | sample | not available | sample_conditions |
| 2D 1H-1H NOESY | sample | not available | sample_conditions |
| 3D HNCACB | sample | not available | sample_conditions |
| HNCA | sample | not available | sample_conditions |
| CBCA (CO) NH | sample | not available | sample_conditions |
| HNCO | sample | not available | sample_conditions |
| HNHA | sample | not available | sample_conditions |
| 3D H (CCO) NH | sample | not available | sample_conditions |
| C (CO) NH | sample | not available | sample_conditions |
| DE-H (C) CH-TOCSY | sample | not available | sample_conditions |
| 3D 1H-15N NOESY | sample | not available | sample_conditions |
| 13C NOESY | sample | not available | sample_conditions |
Software:
No software information available
NMR spectrometers:
- VARIAN INOVA 500 MHz
- VARIAN INOVA 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks