BMRB Entry 6270

Name: 1H, 13C, and 15N Chemical Shift Assignments for chPrP(25-242)
Published: 2004-10-18

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6270

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for chPrP(25-242)

Deposition date:

2004-07-22

Original release date:

2004-10-18

Authors:

Lysek, Dominikus; Calzolai, Luigi; Wuthrich, Kurt

Citation:

Citation: Lysek, Dominikus; Calzolai, Luigi; Wuthrich, Kurt. "Letters to the Editor: NMR assignment of the chicken prion protein fragments chPrP(128-242) and chPrP(25-242)" J. Biomol. NMR 30, 97-97 (2004).
PubMed: 15459548

Assembly members:

Assembly members:
chicken prion protein, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
chicken prion protein: GSKKGKGKPSGGGWGAGSHR QPSYPRQPGYPHNPGYPHNP GYPHNPGYPHNPGYPQNPGY PHNPGYPGWGQGYNPSSGGS YHNQKPWKPPKTNFKHVAGA AAAGAVV

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	160
15N chemical shifts	82
1H chemical shifts	476

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	chPrP (25-242)	1

Entities:

Entity 1, chPrP (25-242) 107 residues - Formula weight is not available

1

GLY

SER

LYS

GLY

LYS

GLY

LYS

PRO

SER

2

GLY

TRP

GLY

ALA

GLY

SER

HIS

ARG

3

GLN

PRO

SER

TYR

PRO

ARG

GLN

PRO

GLY

TYR

4

PRO

HIS

ASN

PRO

GLY

TYR

PRO

HIS

ASN

PRO

5

GLY

TYR

PRO

HIS

ASN

PRO

GLY

TYR

PRO

HIS

6

ASN

PRO

GLY

TYR

PRO

GLN

ASN

PRO

GLY

TYR

7

PRO

HIS

ASN

PRO

GLY

TYR

PRO

GLY

TRP

GLY

8

GLN

GLY

TYR

ASN

PRO

SER

GLY

SER

9

TYR

HIS

ASN

GLN

LYS

PRO

TRP

LYS

PRO

10

LYS

THR

ASN

PHE

LYS

HIS

VAL

ALA

GLY

ALA

11

ALA

GLY

ALA

VAL

Samples:

sample_1: chicken prion protein, [U-30% 13C; U-30% 15N], 1.0 mM; sodium acetate 10 mM

Ex-cond_1: ionic strength: 10 mM; pH: 4.3; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	not available	not available	not available
1H-13N NOESY aliphatic, aromatic	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Bruker DRX 750 MHz