BMRB Entry 6269
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6269
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Lysek, Dominikus; Calzolai, Luigi; Wuthrich, Kurt. "Letters to the Editor: NMR assignment of the chicken prion protein fragments
chPrP(128-242) and chPrP(25-242)" J. Biomol. NMR 30, 97-97 (2004).
PubMed: 15459548
Assembly members:
chicken prion protein, polymer, 117 residues, Formula weight is not available
Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
chicken prion protein: GSVVGGLGGYAMGRVMSGMN
YHFDRPDEYRWWSENSARYP
NRVYYRDYSSPVPQDVFVAD
CFNITVTEYSIGPAAKKNTS
EAVAAANQTEVEMENKVVTK
VIREMCVQQYREYRLAS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 355 |
| 15N chemical shifts | 135 |
| 1H chemical shifts | 804 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | chPrP (128-242) | 1 |
Entities:
Entity 1, chPrP (128-242) 117 residues - Formula weight is not available
| 1 | GLY | SER | VAL | VAL | GLY | GLY | LEU | GLY | GLY | TYR | ||||
| 2 | ALA | MET | GLY | ARG | VAL | MET | SER | GLY | MET | ASN | ||||
| 3 | TYR | HIS | PHE | ASP | ARG | PRO | ASP | GLU | TYR | ARG | ||||
| 4 | TRP | TRP | SER | GLU | ASN | SER | ALA | ARG | TYR | PRO | ||||
| 5 | ASN | ARG | VAL | TYR | TYR | ARG | ASP | TYR | SER | SER | ||||
| 6 | PRO | VAL | PRO | GLN | ASP | VAL | PHE | VAL | ALA | ASP | ||||
| 7 | CYS | PHE | ASN | ILE | THR | VAL | THR | GLU | TYR | SER | ||||
| 8 | ILE | GLY | PRO | ALA | ALA | LYS | LYS | ASN | THR | SER | ||||
| 9 | GLU | ALA | VAL | ALA | ALA | ALA | ASN | GLN | THR | GLU | ||||
| 10 | VAL | GLU | MET | GLU | ASN | LYS | VAL | VAL | THR | LYS | ||||
| 11 | VAL | ILE | ARG | GLU | MET | CYS | VAL | GLN | GLN | TYR | ||||
| 12 | ARG | GLU | TYR | ARG | LEU | ALA | SER |
Samples:
sample_1: chicken prion protein, [U-13C; U-15N], 1.0 mM; sodium acetate 1.0 mM
Ex-cond_1: ionic strength: 10 mM; pH: 4.3; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| 1H-13N NOESY aliphatic, aromatic | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 750 MHz
- Bruker DRX 600 MHz
- Bruker DRX 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks