BMRB Entry 6267

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6267

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Title:
1H, 13C and 15N resonance assignments of the phosphorylated enzyme IIB (C31S) of the mannitol-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli
Deposition date:
2004-07-19
Original release date:
2005-01-07
Authors:
Otten, Renee; Van Lune, Franciska; Dijkstra, Klaas; Scheek, Ruud
Citation:

Citation: Otten, Renee; Van Lune, Franciska; Dijkstra, Klaas; Scheek, Ruud. "Letter to the Editor: 1H, 13C and 15N resonance assignments of the phosphorylated enzyme IIB of the mannitol-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli"  J. Biomol. NMR 30, 461-462 (2004).

Assembly members:

Assembly members:
phospho-IIB-mannitol, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
phospho-IIB-mannitol: GSHMSHVRKIIVASDAGMGS SAMGAGVLRKKIQDAGLSQI SVTNSAINNLPPDVDLVITH RDLTERAMRQVPQAQHISLT NFLDSGLYTSLTERLVAAQR HTANEEKVKDSLKDSFD

Data sets:
Data typeCount
1H chemical shifts737
13C chemical shifts453
15N chemical shifts120

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1phospho-IIB-mannitol1

Entities:

Entity 1, phospho-IIB-mannitol 117 residues - Formula weight is not available

1   GLYSERHISMETSERHISVALARGLYSILE
2   ILEVALALASERASPALAGLYMETGLYSER
3   SERALAMETGLYALAGLYVALLEUARGLYS
4   LYSILEGLNASPALAGLYLEUSERGLNILE
5   SERVALTHRASNSERALAILEASNASNLEU
6   PROPROASPVALASPLEUVALILETHRHIS
7   ARGASPLEUTHRGLUARGALAMETARGGLN
8   VALPROGLNALAGLNHISILESERLEUTHR
9   ASNPHELEUASPSERGLYLEUTYRTHRSER
10   LEUTHRGLUARGLEUVALALAALAGLNARG
11   HISTHRALAASNGLUGLULYSVALLYSASP
12   SERLEULYSASPSERPHEASP

Samples:

sample: phospho-IIB-mannitol, [U-13C; U-15N], 2.0 mM; Tris-Acetate buffer 20 mM; DTT 3 mM; NaN3 1 mM; D2O 5%; EDTA 1 mM

Ex-cond_1: pH: 6.2; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
[15N,1H]-cpmg-HSQCsamplenot availableEx-cond_1
HNCAsamplenot availableEx-cond_1
HN(CO)CAsamplenot availableEx-cond_1
HN(CA)COsamplenot availableEx-cond_1
HNCOsamplenot availableEx-cond_1
HNCACBsamplenot availableEx-cond_1
CBCA(CO)NHsamplenot availableEx-cond_1
COCAHsamplenot availableEx-cond_1
HC(C)H-TOCSYsamplenot availableEx-cond_1
HBHA(CBCACO)NHsamplenot availableEx-cond_1
CBCGCDCEHEsamplenot availableEx-cond_1
CBCGCDHDsamplenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian Unity INOVA 600 MHz

Related Database Links:

PDB
DBJ BAE77694 BAG79401 BAJ45339 BAL40192
EMBL CAA24748 CAQ33922 CAQ91062 CAR00566 CAV00578
GB AAB18576 AAC76623 ABV08013 ACB04648 ACR63207
REF NP_418056 WP_000093235 WP_000093236 WP_000093240 WP_000093243
SP P00550
AlphaFold P00550

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks