BMRB Entry 6265
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6265
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Andersen, P.; Kragelund, B.; Olsen, A.; Larsen, F.; Chua, N.-H.; Poulsen, F.; Skriver, K.. "Structure and Biochemical Function of a Prototypical Arabidopsis U-box Domain" J. Biol. Chem. 279, 40053-40061 (2004).
PubMed: 15231834
Assembly members:
PUB14, polymer, 78 residues, Formula weight is not available
Natural source: Common Name: thale cress Taxonomy ID: 8754 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PGEX-4T-1
Entity Sequences (FASTA):
PUB14: GSPEFPEYFRCPISLELMKD
PVIVSTGQTYERSSIQKWLD
AGHKTCPKSQETLLHAGLTP
NYVLKSLIALWCESNGIE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 256 |
| 15N chemical shifts | 63 |
| 1H chemical shifts | 498 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PUB14 | 1 |
Entities:
Entity 1, PUB14 78 residues - Formula weight is not available
| 1 | GLY | SER | PRO | GLU | PHE | PRO | GLU | TYR | PHE | ARG | ||||
| 2 | CYS | PRO | ILE | SER | LEU | GLU | LEU | MET | LYS | ASP | ||||
| 3 | PRO | VAL | ILE | VAL | SER | THR | GLY | GLN | THR | TYR | ||||
| 4 | GLU | ARG | SER | SER | ILE | GLN | LYS | TRP | LEU | ASP | ||||
| 5 | ALA | GLY | HIS | LYS | THR | CYS | PRO | LYS | SER | GLN | ||||
| 6 | GLU | THR | LEU | LEU | HIS | ALA | GLY | LEU | THR | PRO | ||||
| 7 | ASN | TYR | VAL | LEU | LYS | SER | LEU | ILE | ALA | LEU | ||||
| 8 | TRP | CYS | GLU | SER | ASN | GLY | ILE | GLU |
Samples:
sample_1: PUB14, [U-15N; U-13C], 0.9 mM; sodium phosphate 20 mM; NaCl 0.15 M; DTT 1 mM; D20 10%; H20 90%
sample_2: PUB14, [U-15N; U-13C], 0.6 mM; sodium phosphate 20 mM; NaCl 0.15 M; DTT 1 mM; D20 10%; H20 90%
sample_3: PUB14, [U-15N], 0.6 mM; sodium phosphate 20 mM; NaCl 0.15 M; DTT 1 mM; D20 10%; H20 90%
sample_4: PUB14, [U-15N; U-13C], 0.4 mM; sodium phosphate 20 mM; NaCl 0.15 M; DTT 1 mM; D20 99.96%; H20 0.04%
sample_cond_1: ionic strength: 0.17 M; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| (HB)CB(CGCD)HD | not available | not available | not available |
| 3D 15N-separated TOCSY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 13C-separated TOCSY | not available | not available | not available |
| 3D 13C-separated NOESY | not available | not available | not available |
Software:
NMRPipe - processing
PRONTO v20020517 - data analysis
CYANA - structure calculation
X-PLOR vV3.840 - structure calculation
CNS v1.1 - refinement
NMR spectrometers:
- Varian UNITY INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAH57001 |
| GB | AAL38755 AAM20180 AEE79300 EFH52534 |
| REF | NP_191045 XP_002876275 XP_010427209 XP_010504312 XP_010516027 |
| SP | Q8VZ40 |
| AlphaFold | Q8VZ40 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks