BMRB Entry 6264
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6264
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Jing, W.; Hunter, H.; Tanabe, H.; Ouellette, A.; Vogel, H.. "Solution structure of cryptdin-4, a mouse paneth cell alpha-defensin" Biochemistry 43, 15759-15766 (2004).
PubMed: 15595831
Assembly members:
Cryptdin-4, polymer, 32 residues, Formula weight is not available
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET-28A
Entity Sequences (FASTA):
Cryptdin-4: GLLCYCRKGHCKRGERVRGT
CGIRFLYCCPRR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 233 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Cryptdin-4 | 1 |
Entities:
Entity 1, Cryptdin-4 32 residues - Formula weight is not available
| 1 | GLY | LEU | LEU | CYS | TYR | CYS | ARG | LYS | GLY | HIS | ||||
| 2 | CYS | LYS | ARG | GLY | GLU | ARG | VAL | ARG | GLY | THR | ||||
| 3 | CYS | GLY | ILE | ARG | PHE | LEU | TYR | CYS | CYS | PRO | ||||
| 4 | ARG | ARG |
Samples:
sample_1: Cryptdin-4 3.2 mg
sample_cond_1: pH: 4.2; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
Software:
NMRView v4.1.3 - data analysis
CNS - refinement
ARIA v1.2 - structure solution
NMR spectrometers:
- Bruker Advance 700 MHz