BMRB Entry 6260

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6260
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Title:
NMR solution Structure of a Highly Stable de novo Heterodimeric Coiled-Coil
Deposition date:
2004-07-12
Original release date:
2004-07-26
Authors:
Lindhout, Darrin; Litowski, Jennifer; Mercier, Pascal; Hodges, Robert; Sykes, Brian
Citation:

Citation: Lindhout, Darrin; Litowski, Jennifer; Mercier, Pascal; Hodges, Robert; Sykes, Brian. "NMR solution Structure of a Highly Stable de novo Heterodimeric Coiled-Coil"  Biopolymers ., .-..

Assembly members:

Assembly members:
IAAL-E3, polymer, 21 residues, Formula weight is not available
K3 coiled-coil, polymer, 21 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IAAL-E3: EIAALEKEIAALEKEIAALE K
K3 coiled-coil: KIAALKEKIAALKEKIAALK E

Data sets:
Data typeCount
15N chemical shifts42
1H chemical shifts240

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IAAL-E31
2K3 coiled-coil2

Entities:

Entity 1, IAAL-E3 21 residues - Formula weight is not available

1   GLUILEALAALALEUGLULYSGLUILEALA
2   ALALEUGLULYSGLUILEALAALALEUGLU
3   LYS

Entity 2, K3 coiled-coil 21 residues - Formula weight is not available

1   LYSILEALAALALEULYSGLULYSILEALA
2   ALALEULYSGLULYSILEALAALALEULYS
3   GLU

Samples:

sample_1: IAAL-E3 1.0 mM; K3 coiled-coil 1.0 mM

experimental: ionic strength: 150 mM; pH: 6.70; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-1H NOESYnot availablenot availablenot available
1H-1H TOCSYnot availablenot availablenot available

Software:

VNMR -

CYANA -

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
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