BMRB Entry 6259

Name: 1H, 13C, and 15N chemical shift assignments for GatB (backbone + side chains)
Published: 2006-10-02

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PDB ID: 1tvm
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6259
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N chemical shift assignments for GatB (backbone + side chains)

Deposition date:

2004-07-02

Original release date:

2006-10-02

Authors:

VOLPON, Laurent; YOUNG, Chris; GEHRING, Kalle

Citation:

Citation: Volpon, Laurent; Young, Chris; Matte, A.; Gehring, Kalle. "NMR structure of the enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system and its interaction with GatA." Protein Sci. 15, 2435-2441 (2006).
PubMed: 16963640

Assembly members:

Assembly members:
GatB, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GatB: MGSSHHHHHHHHENLYFQGS KRKIIVACGGAVATSTMAAE EIKELCQSHNIPVELIQCRV NEIETYMDGVHLICTTARVD RSFGDIPLVHGMPFVSGVGI EALQNKILTILQG

Data sets:

assigned_chemical_shifts
- chemical_shifts

Data type	Count
13C chemical shifts	412
1H chemical shifts	614
15N chemical shifts	99

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GatB monomer	1

Entities:

Entity 1, GatB monomer 113 residues - Formula weight is not available

1

MET

GLY

SER

HIS

2

HIS

GLU

ASN

LEU

TYR

PHE

GLN

GLY

SER

3

LYS

ARG

LYS

ILE

VAL

ALA

CYS

GLY

4

ALA

VAL

ALA

THR

SER

THR

MET

ALA

GLU

5

GLU

ILE

LYS

GLU

LEU

CYS

GLN

SER

HIS

ASN

6

ILE

PRO

VAL

GLU

LEU

ILE

GLN

CYS

ARG

VAL

7

ASN

GLU

ILE

GLU

THR

TYR

MET

ASP

GLY

VAL

8

HIS

LEU

ILE

CYS

THR

ALA

ARG

VAL

ASP

9

ARG

SER

PHE

GLY

ASP

ILE

PRO

LEU

VAL

HIS

10

GLY

MET

PRO

PHE

VAL

SER

GLY

VAL

GLY

ILE

11

GLU

ALA

LEU

GLN

ASN

LYS

ILE

LEU

THR

ILE

12

LEU

GLN

GLY

Samples:

sample_1: GatB, [U-13C; U-15N], 1.0 mM; KCl 75 mM; NaCl 75 mM

Ex-cond_1: pH: 6.8; temperature: 298 K; pressure: 1 atm; ionic strength: 0.15 M

Experiments:

Name	Sample	Sample state	Sample conditions
(HCCH) TOCSY	sample_1	not available	Ex-cond_1
HNCACB	sample_1	not available	Ex-cond_1
CBCA(CO)NH	sample_1	not available	Ex-cond_1
2D NOESY	sample_1	not available	Ex-cond_1

Software:

Sparky v3.106 -

NMR spectrometers:

Bruker AVANCE 600 MHz

PDB	1TVM
DBJ	BAB36319 BAG77886 BAH46969 BAI31343 BAI55515
EMBL	CAA56229 CAR03521 CAR13613 CAR17061 CAS09786
GB	AAG57150 AAN43688 AAN81074 AAP17514 ABB62344
PRF	2113201D
REF	NP_288595 NP_310923 NP_707981 NP_754506 NP_837705
SP	P0A435 P0A436 P0A437
AlphaFold	P0A435 P0A436 P0A437