BMRB Entry 6257
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6257
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Citation: Laurents, Douglas; Gorman, Paul; Guo, Ming; Rico, Manuel; Chakrabartty, Avijit; Bruix, Marta. "Alzheimer's Abeta40 Studied by NMR at Low pH Reveals That DSS Binds and Promotes
beta-ball Oligomerization" J. Biol. Chem. 280, 3675-3685 (2004).
PubMed: 15557279
Assembly members:
Abeta(1-40), polymer, 40 residues, 4300 Da.
Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Abeta(1-40): DAEFRHDSGYEVHHQKLVFF
AEDVGSNKGAIIGLMVGGVV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 97 |
| 1H chemical shifts | 279 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Abeta(1-40) | 1 |
Entities:
Entity 1, Abeta(1-40) 40 residues - 4300 Da.
| 1 | ASP | ALA | GLU | PHE | ARG | HIS | ASP | SER | GLY | TYR | |
| 2 | GLU | VAL | HIS | HIS | GLN | LYS | LEU | VAL | PHE | PHE | |
| 3 | ALA | GLU | ASP | VAL | GLY | SER | ASN | LYS | GLY | ALA | |
| 4 | ILE | ILE | GLY | LEU | MET | VAL | GLY | GLY | VAL | VAL |
Samples:
sample_1: Abeta(1-40)0.15 – 0.30 mM; NaH2PO4 5 mM
Ex-cond_1: ionic strength: 0.01 M; pH: 2.5; temperature: 298 K
Ex-cond_2: ionic strength: 0.01 M; pH*: 2.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
| COSY | not available | not available | not available |
| 13C-HSQC | not available | not available | not available |
Software:
UXWINNMR -
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 5400 6554 |
| PDB | |
| DBJ | BAA22264 BAA84580 BAD51938 BAE01907 BAG10647 |
| EMBL | CAA30050 CAA31830 CAA39589 CAA39590 CAA39591 |
| GenBank | AAA35540 AAA36829 AAA51564 AAA51722 AAA51726 |
| PIR | A60045 D60045 E60045 G60045 PQ0438 |
| PRF | 1303338A 1403400A 1405204A 1507304A 1507304B |
| REF | NP_000475 NP_001006601 NP_001013036 NP_001070264 NP_958816 |
| SWISS-PROT | P05067 P53601 P79307 Q28053 Q28280 |