BMRB Entry 6253

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6253

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Title:
1H, 13C and 15N resonance assignment of human gamma S-crystallin, a 21-kDa eye-lens protein
Deposition date:
2004-06-28
Original release date:
2004-12-16
Authors:
Baraguey, Carine; Skouri-Panet, Feriel; Tardieu, Annette; Chassaing, Gerard; Lequin, Olivier
Citation:

Citation: Baraguey, Carine; Skouri-Panet, Feriel; Bontems, Francois; Tardieu, Annette; Chassaing, Gerard; Lequin, Olivier. "Letter to the Editor: 1H, 15N and 13C resonance assignment of human gamma S- crystallin, a 21-kDa eye-lens protein"  J. Biomol. NMR 30, 385-386 (2004).

Assembly members:

Assembly members:
single chain polypeptide, polymer, 177 residues, 20859.5 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
single chain polypeptide: SKTGTKITFYEDKNFQGRRY DCDSDCADFHTYLSRCNSIK VEGGTWAVYERPNFAGYMYI LPQGEYPEYQRWMGLNDRLS SCRAVHLPSGGQYKIQIFEK GDFSGQMYETTEDCPSIMEQ FHMREIHSCKVLEGVWIFYE LPNYRGRQYLLDKKEYRKPI DWGAASPAVQSFRRIVE

Data sets:
Data typeCount
13C chemical shifts761
15N chemical shifts195
1H chemical shifts1057

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gamma S-crystallin1

Entities:

Entity 1, gamma S-crystallin 177 residues - 20859.5 Da.

1   SERLYSTHRGLYTHRLYSILETHRPHETYR
2   GLUASPLYSASNPHEGLNGLYARGARGTYR
3   ASPCYSASPSERASPCYSALAASPPHEHIS
4   THRTYRLEUSERARGCYSASNSERILELYS
5   VALGLUGLYGLYTHRTRPALAVALTYRGLU
6   ARGPROASNPHEALAGLYTYRMETTYRILE
7   LEUPROGLNGLYGLUTYRPROGLUTYRGLN
8   ARGTRPMETGLYLEUASNASPARGLEUSER
9   SERCYSARGALAVALHISLEUPROSERGLY
10   GLYGLNTYRLYSILEGLNILEPHEGLULYS
11   GLYASPPHESERGLYGLNMETTYRGLUTHR
12   THRGLUASPCYSPROSERILEMETGLUGLN
13   PHEHISMETARGGLUILEHISSERCYSLYS
14   VALLEUGLUGLYVALTRPILEPHETYRGLU
15   LEUPROASNTYRARGGLYARGGLNTYRLEU
16   LEUASPLYSLYSGLUTYRARGLYSPROILE
17   ASPTRPGLYALAALASERPROALAVALGLN
18   SERPHEARGARGILEVALGLU

Samples:

sample_1: single chain polypeptide, [U-15N; U-13C], 1 – 2 mM; sodium phosphate 13.3 mM; KCl 30 mM; EDTA 0.3 mM; DTT 0.3 mM; DSS 0.1 mM; NaN3 0.02 % (w/v)

conditions_1: ionic strength: 0.05 M; pH: 6; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESY-HSQCnot availablenot availablenot available
3D 1H-15N TOCSY-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
3D 1H-13C NOESY-HMQCnot availablenot availablenot available
(HB)CB(CGCD)HDnot availablenot availablenot available

Software:

xwinnmr v2.6 -

XEASY -

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 17576 17582
PDB
DBJ BAG36855
GB AAD45901 AAF72490 AAH69478 AAH70241 ADQ32938
REF NP_060011 XP_001092090 XP_002758251 XP_003256655 XP_003894751
SP P22914
AlphaFold P22914

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks