BMRB Entry 6247

Name: Solution structure of C-terminal domain of NifU-like protein from Oryza sativa
Published: 2005-10-20

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PDB ID: 1th5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6247
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of C-terminal domain of NifU-like protein from Oryza sativa

Deposition date:

2004-06-23

Original release date:

2005-10-20

Authors:

Kumeta, H.; Ogura, K.; Asayama, M.; Katoh, S.; Katoh, E.; Inagaki, F.

Citation:

Citation: Katoh, S.; Murata, K.; Kubota, Y.; Kumeta, H.; Ogura, K.; Inagaki, F.; Asayama, M.; Katoh, E.. "Refolding and purification of recombinant OsNifU1A domain II that was expressed by Escherichia coli." Protein Expr. Purif. 43, 149-156 (2005).
PubMed: 15978837

Assembly members:

Assembly members:
NifU1, polymer, 74 residues, Formula weight is not available

Natural source:

Natural source: Common Name: rice Taxonomy ID: 4530 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Oryza sativa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PDEST32A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NifU1: MLELNEENVEKVLNEIRPYL AGTGGGGLQFLMIKGPIVKV RLTGPAAVVRTVRIAVSKKL REKIPSIQIVQLLS

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	344
15N chemical shifts	80
1H chemical shifts	547

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NifU1	1

Entities:

Entity 1, NifU1 74 residues - Formula weight is not available

1

MET

LEU

GLU

LEU

ASN

GLU

ASN

VAL

GLU

2

LYS

VAL

LEU

ASN

GLU

ILE

ARG

PRO

TYR

LEU

3

ALA

GLY

THR

GLY

LEU

GLN

PHE

4

LEU

MET

ILE

LYS

GLY

PRO

ILE

VAL

LYS

VAL

5

ARG

LEU

THR

GLY

PRO

ALA

VAL

ARG

6

THR

VAL

ARG

ILE

ALA

VAL

SER

LYS

LEU

7

ARG

GLU

LYS

ILE

PRO

SER

ILE

GLN

ILE

VAL

8

GLN

LEU

SER

Samples:

sample_1: NifU1, [U-15N], 0.8 mM; NaPi 20 mM; NaCl 100 mM; beta-ME 2.5 mM; H2O 90%; D2O 10%

sample_2: NifU1, [U-13C; U-15N], 0.45 mM; NaPi 20 mM; NaCl 100 mM; beta-ME 2.5 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 100 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	not available	not available	not available
3D 13C-separated NOESY	not available	not available	not available

Software:

VNMR v6.1c - collection

NMRPipe v23 - processing

SPARKY v3.11 - data analysis

CYANA v1.06 - refinement, structure solution

NMR spectrometers:

Varian INOVA 600 MHz