BMRB Entry 6244

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6244
MolProbity Validation Chart

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Title:
Novel all alpha-helical fold for gene target gi3844938 from Mycoplasma genitalium
Deposition date:
2004-06-22
Original release date:
2004-08-04
Authors:
Shi, Jianxia; Pelton, Jeffrey; Wemmer, David
Citation:

Citation: Shi, Jianxia; Pelton, Jeffrey; Yokota, Hisao; Kim, Rosalind; Wemmer, David. "Novel all alpha-helical fold for gene target gi3844938 from Mycoplasma genitalium "  .

Assembly members:

Assembly members:
gi3844938, polymer, 137 residues, 15703 Da.

Natural source:

Natural source:   Common Name: Mycoplasma genitalium   Taxonomy ID: 243273   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Mycoplasma genitalium

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
gi3844938: MEQNNIKEQLISFFNQACST HQERLDFICSTRESDTFSSV DVPLEPIKNIIEITKDENQQ IEITKIAVNNIKTLSSVGAT GQYMASFFSTNSEPAIIFCV IYFLYHFGFLKDNNKKQIIK KAYETIADNIADYLNEN

Data sets:
Data typeCount
1H chemical shifts812
13C chemical shifts557
15N chemical shifts132

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1gi38449381

Entities:

Entity 1, gi3844938 137 residues - 15703 Da.

1   METGLUGLNASNASNILELYSGLUGLNLEU
2   ILESERPHEPHEASNGLNALACYSSERTHR
3   HISGLNGLUARGLEUASPPHEILECYSSER
4   THRARGGLUSERASPTHRPHESERSERVAL
5   ASPVALPROLEUGLUPROILELYSASNILE
6   ILEGLUILETHRLYSASPGLUASNGLNGLN
7   ILEGLUILETHRLYSILEALAVALASNASN
8   ILELYSTHRLEUSERSERVALGLYALATHR
9   GLYGLNTYRMETALASERPHEPHESERTHR
10   ASNSERGLUPROALAILEILEPHECYSVAL
11   ILETYRPHELEUTYRHISPHEGLYPHELEU
12   LYSASPASNASNLYSLYSGLNILEILELYS
13   LYSALATYRGLUTHRILEALAASPASNILE
14   ALAASPTYRLEUASNGLUASN

Samples:

sample_1: gi3844938, [U-93% 13C; U-98% 15N], 1.0 mM; KPO4 25 mM; NaCl 100 mM; EDTA 1 mM; TCEP 1 mM

Ex-cond_1: pH: 6.7 na; temperature: 298 K; ionic strength: 0.14 M

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCACOnot availablenot availablenot available
HACANHnot availablenot availablenot available
hC CONHnot availablenot availablenot available
Hc CONHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
15N-NOESY-HSQCnot availablenot availablenot available
15N-TOCSY-HSQCnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D 13C Double half-filtered TOCSYnot availablenot availablenot available
2D 13C Dboule half-filtered NOESYnot availablenot availablenot available

Software:

NMRView v5.0.4 - Data analysis

nmrPipe v2.1 - Data processing

NMR spectrometers:

  • DRX Bruker 500 MHz
  • DRX Bruker 600 MHz

Related Database Links:

PDB
GB AAC71579 ABY79523 AFQ03197 AFQ03682 AFQ04187
REF NP_073025 WP_009885812 YP_006599905 YP_006600390 YP_006600895
SP P47596
AlphaFold P47596

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks