BMRB Entry 6240
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR6240
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Cornilescu, Claudia; Cornilescu, Gabriel; Singh, Shanteri; Lee, Min; Tyler, Ejan; Shahan, Mark; Vinarov, Dmitriy; Markley, John. "Solution structure of At5g66040, a putative protein from Arabidopsis Thaliana" J. Biomol. NMR ., .-..
Assembly members:
At5g66040, polymer, 129 residues, 13802.3 Da.
Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
At5g66040: MGHHHHHHLEAEESRVPSSV
SVTVAHDLLLAGHRYLDVRT
PEEFSQGHACGAINVPYMNR
GASGMSKNTDFLEQVSSHFG
QSDNIIVGCQSGGRSIKATT
DLLHAGFTGVKDIVGGYSAW
AKNGLPTKA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 614 |
| 13C chemical shifts | 413 |
| 15N chemical shifts | 106 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | At5g66040 | 1 |
Entities:
Entity 1, At5g66040 129 residues - 13802.3 Da.
| 1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | LEU | GLU | ||||
| 2 | ALA | GLU | GLU | SER | ARG | VAL | PRO | SER | SER | VAL | ||||
| 3 | SER | VAL | THR | VAL | ALA | HIS | ASP | LEU | LEU | LEU | ||||
| 4 | ALA | GLY | HIS | ARG | TYR | LEU | ASP | VAL | ARG | THR | ||||
| 5 | PRO | GLU | GLU | PHE | SER | GLN | GLY | HIS | ALA | CYS | ||||
| 6 | GLY | ALA | ILE | ASN | VAL | PRO | TYR | MET | ASN | ARG | ||||
| 7 | GLY | ALA | SER | GLY | MET | SER | LYS | ASN | THR | ASP | ||||
| 8 | PHE | LEU | GLU | GLN | VAL | SER | SER | HIS | PHE | GLY | ||||
| 9 | GLN | SER | ASP | ASN | ILE | ILE | VAL | GLY | CYS | GLN | ||||
| 10 | SER | GLY | GLY | ARG | SER | ILE | LYS | ALA | THR | THR | ||||
| 11 | ASP | LEU | LEU | HIS | ALA | GLY | PHE | THR | GLY | VAL | ||||
| 12 | LYS | ASP | ILE | VAL | GLY | GLY | TYR | SER | ALA | TRP | ||||
| 13 | ALA | LYS | ASN | GLY | LEU | PRO | THR | LYS | ALA |
Samples:
sample_1: At5g66040, [U-13C; U-15N], 1 mM
sample_cond: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-HSQC | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCACONH | not available | not available | not available |
| HNCO | not available | not available | not available |
| HBHACONH | not available | not available | not available |
| HCCONH | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| 3D-15N NOESY | not available | not available | not available |
| 3D-13C NOESY | not available | not available | not available |
| 15N-T2 | not available | not available | not available |
Software:
nmrPipe - data processing, automated peak picking
PIPP/STAPP - manual peak assignments
NMR spectrometers:
- Varian INOVA 600 MHz
- Bruker AVANCE 750 MHz
- Bruker AVANCE 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAB10409 |
| GB | AAK83644 AAM61332 AAN38701 AED98145 AED98146 |
| REF | NP_569026 NP_851278 |
| SP | Q39129 |
| AlphaFold | Q39129 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks