BMRB Entry 6238

Title:
Complete 1H, 15N amd 13C Chemical Shift Assignments of the murine CBP ZZ domain (a.a. 1700-1751)   PubMed: 15476823
Deposition date:
2004-06-14
Original release date:
2004-11-12
Authors:
Legge, Glen; Hambly, David; Trinh, Tam; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter
Citation:

Citation: Legge, Glen; Martinez-Yamout, Maria; Hambly, David; Trinh, Tam; Lee, B.; Dyson, H.; Wright, Peter. "ZZ Domain of CBP: an Unusual Zinc Finger Fold in a Protein Interaction Module"  J. Mol. Biol. 343, 1081-1093 (2004).

Assembly members:

Assembly members:
ZZ, polymer, 52 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Experimental source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts222
1H chemical shifts353
15N chemical shifts56

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ZZ domain of CBP1
2ZINC (II) ION, 12
3ZINC (II) ION, 22

Entities:

Entity 1, ZZ domain of CBP 52 residues - Formula weight is not available

1   GLYGLNASPARGPHEVALTYRTHRCYSASN
2   GLUCYSLYSHISHISVALGLUTHRARGTRP
3   HISCYSTHRVALCYSGLUASPTYRASPLEU
4   CYSILEASNCYSTYRASNTHRLYSSERHIS
5   THRHISLYSMETVALLYSTRPGLYLEUGLY
6   LEUASP

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: ZZ, [U-13C; U-15N], 2.3 mM; Zinc Chloride 50 uM; Salt 150 mM; Tris 20 mM; DTT 2 mM; D2O 5 % v/v

Ex-cond_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
CCONH TOCSYsample_1not availableEx-cond_1
HCCONH TOCSYsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
HCCH TOCSYsample_1not availableEx-cond_1
HCCH COSYsample_1not availableEx-cond_1
HNHBsample_1not availableEx-cond_1
CN NOESYsample_1not availableEx-cond_1
1H/15N NOESY HSQCsample_1not availableEx-cond_1
1H13C HSQCsample_1not availableEx-cond_1
1H13C HSQC aromaticsample_1not availableEx-cond_1
CGCOsample_1not availableEx-cond_1
CGCNsample_1not availableEx-cond_1
CBCGCDsample_1not availableEx-cond_1
HNHAsample_1not availableEx-cond_1
1H15H TOSCY HSQCsample_1not availableEx-cond_1

Software:

NMRPIPE, Ad Bax -

NMRVIEW v5.04, One Moon Scientific -

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAE06125 BAI45616
GB AAB28651 AAC17736 AAC51331 AAC51770 AAI72737
PRF 1923401A
REF NP_001020603 NP_001073315 NP_001157494 NP_001247644 NP_004371
SP P45481 Q6JHU9 Q92793
TPG DAA15549
AlphaFold Q92793

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks