BMRB Entry 6238

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PDB ID: 1tot
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6238
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Complete 1H, 15N amd 13C Chemical Shift Assignments of the murine CBP ZZ domain (a.a. 1700-1751)

Deposition date:

2004-06-14

Original release date:

2004-11-12

Authors:

Legge, Glen; Hambly, David; Trinh, Tam; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter

Citation:

Citation: Legge, Glen; Martinez-Yamout, Maria; Hambly, David; Trinh, Tam; Lee, B.; Dyson, H.; Wright, Peter. "ZZ Domain of CBP: an Unusual Zinc Finger Fold in a Protein Interaction Module" J. Mol. Biol. 343, 1081-1093 (2004).
PubMed: 15476823

Assembly members:

Assembly members:
ZZ, polymer, 52 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ZZ: GQDRFVYTCNECKHHVETRW HCTVCEDYDLCINCYNTKSH THKMVKWGLGLD

Data sets:

assigned_chemical_shifts
- shift_1

Data type	Count
13C chemical shifts	222
1H chemical shifts	353
15N chemical shifts	56

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ZZ domain of CBP	1
2	ZINC (II) ION, 1	2
3	ZINC (II) ION, 2	2

Entities:

Entity 1, ZZ domain of CBP 52 residues - Formula weight is not available

1

GLY

GLN

ASP

ARG

PHE

VAL

TYR

THR

CYS

ASN

2

GLU

CYS

LYS

HIS

VAL

GLU

THR

ARG

TRP

3

HIS

CYS

THR

VAL

CYS

GLU

ASP

TYR

ASP

LEU

4

CYS

ILE

ASN

CYS

TYR

ASN

THR

LYS

SER

HIS

5

THR

HIS

LYS

MET

VAL

LYS

TRP

GLY

LEU

GLY

6

LEU

ASP

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: ZZ, [U-13C; U-15N], 2.3 mM; Zinc Chloride 50 uM; Salt 150 mM; Tris 20 mM; DTT 2 mM; D2O 5 % v/v

Ex-cond_1: pH: 6.8; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N HSQC	sample_1	not available	Ex-cond_1
HNCA	sample_1	not available	Ex-cond_1
CCONH TOCSY	sample_1	not available	Ex-cond_1
HCCONH TOCSY	sample_1	not available	Ex-cond_1
CBCA(CO)NH	sample_1	not available	Ex-cond_1
HCCH TOCSY	sample_1	not available	Ex-cond_1
HCCH COSY	sample_1	not available	Ex-cond_1
HNHB	sample_1	not available	Ex-cond_1
CN NOESY	sample_1	not available	Ex-cond_1
1H/15N NOESY HSQC	sample_1	not available	Ex-cond_1
1H13C HSQC	sample_1	not available	Ex-cond_1
1H13C HSQC aromatic	sample_1	not available	Ex-cond_1
CGCO	sample_1	not available	Ex-cond_1
CGCN	sample_1	not available	Ex-cond_1
CBCGCD	sample_1	not available	Ex-cond_1
HNHA	sample_1	not available	Ex-cond_1
1H15H TOSCY HSQC	sample_1	not available	Ex-cond_1

Software:

NMRPIPE, Ad Bax -

NMRVIEW v5.04, One Moon Scientific -

NMR spectrometers:

Bruker AMX 500 MHz
Bruker DRX 600 MHz

PDB	1TOT
DBJ	BAE06125 BAI45616
GB	AAB28651 AAC17736 AAC51331 AAC51770 AAI72737
PRF	1923401A
REF	NP_001020603 NP_001073315 NP_001157494 NP_001247644 NP_004371
SP	P45481 Q6JHU9 Q92793
TPG	DAA15549
AlphaFold	P45481 Q6JHU9 Q92793