BMRB Entry 6233

Name: (poly)ubiquitin-binding region of S5a
Published: 2005-03-21

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PDB ID: 1yx4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6233
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

(poly)ubiquitin-binding region of S5a

Deposition date:

2004-06-10

Original release date:

2005-03-21

Authors:

Wang, Qinghua; Walters, Kylie

Citation:

Citation: Wang, Qinghua; Walters, Kylie. "Letter to the Editor: Chemical shift assignments of the (poly)ubiquitin-binding region of the proteasome subunit S5a" J. Biomol. NMR 30, 231-232 (2004).

Assembly members:

Assembly members:
(poly)ubiquitin-binding region of S5a, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
(poly)ubiquitin-binding region of S5a: MLGLGASDFEFGVDPSADPE LALALRVSMEEQRQRQEEEA RRAAAASAAEAGIATTGTED SDDALLKMTISQQEFGRTGL PDLSSMTEEEQIAYAMQMSL QGAEFGQAESA

Data sets:

assigned_chemical_shifts
- chemical_shfit_list1

Data type	Count
1H chemical shifts	636
13C chemical shifts	417
15N chemical shifts	117

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	S5a	1

Entities:

Entity 1, S5a 111 residues - Formula weight is not available

1

MET

LEU

GLY

LEU

GLY

ALA

SER

ASP

PHE

GLU

2

PHE

GLY

VAL

ASP

PRO

SER

ALA

ASP

PRO

GLU

3

LEU

ALA

LEU

ALA

LEU

ARG

VAL

SER

MET

GLU

4

GLU

GLN

ARG

GLN

ARG

GLN

GLU

ALA

5

ARG

ALA

SER

ALA

GLU

6

ALA

GLY

ILE

ALA

THR

GLY

THR

GLU

ASP

7

SER

ASP

ALA

LEU

LYS

MET

THR

ILE

8

SER

GLN

GLU

PHE

GLY

ARG

THR

GLY

LEU

9

PRO

ASP

LEU

SER

MET

THR

GLU

10

GLN

ILE

ALA

TYR

ALA

MET

GLN

MET

SER

LEU

11

GLN

GLY

ALA

GLU

PHE

GLY

GLN

ALA

GLU

SER

12

ALA

Samples:

sample1: (poly)ubiquitin-binding region of S5a, [U-13C; U-15N], 0.35 mM

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	sample1	not available	Ex-cond_1
HNCO	sample1	not available	Ex-cond_1
TOCSY-15N-HSQC	sample1	not available	Ex-cond_1
15N-HSQC	sample1	not available	Ex-cond_1
15-NOESY	sample1	not available	Ex-cond_1

Software:

No software information available

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 600 MHz

PDB	1YX4 1YX5 1YX6 2KDE 2KDF
DBJ	BAA97576 BAA97577 BAA97581 BAI45716
GB	AAB54057 AAB68598 AAC50433 AAG09200 AAH02365
PRF	2207223A
REF	NP_001013616 NP_001038000 NP_001098398 NP_001130032 NP_001130036
SP	P55036 Q58DA0
TPG	DAA31643
AlphaFold	P55036 Q58DA0 P55036 Q58DA0 P55036 Q58DA0