BMRB Entry 6232

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6232

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Title:
TM1442-PO4
Deposition date:
2004-06-10
Original release date:
2007-03-22
Authors:
Etezady-Esfarjani, Touraj; Placzek, William; Herrmann, Torsten; Wuthrich, Kurt
Citation:

Citation: Etezady-Esfarjani, Touraj; Placzek, William; Herrmann, Torsten; Wuthrich, Kurt. "Solution structures of the putative anti-sigma-factor antagonist TM1442 from Thermotoga maritima in the free and phosphorylated states."  Magn Reson Chem. 44, S61-S70 (2006).
PubMed: 16826544

Assembly members:

Assembly members:
anti-sigma-factor antagonist, polymer, 110 residues, Formula weight is not available
SEP, non-polymer, 185.072 Da.

Natural source:

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
anti-sigma-factor antagonist: MNNLKLDIVEQDDKAIVRVQ GDIDAYNSSELKEQLRNFIS TTSKKKIVLDLSSVSYMDXA GLGTLVVILKDAKINGKEFI LSSLKESISRILKLTHLDKI FKITDTVEEA

Data sets:
Data typeCount
1H chemical shifts174
13C chemical shifts199
15N chemical shifts103

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tm1442 monomer1

Entities:

Entity 1, Tm1442 monomer 110 residues - Formula weight is not available

1   METASNASNLEULYSLEUASPILEVALGLU
2   GLNASPASPLYSALAILEVALARGVALGLN
3   GLYASPILEASPALATYRASNSERSERGLU
4   LEULYSGLUGLNLEUARGASNPHEILESER
5   THRTHRSERLYSLYSLYSILEVALLEUASP
6   LEUSERSERVALSERTYRMETASPSEPALA
7   GLYLEUGLYTHRLEUVALVALILELEULYS
8   ASPALALYSILEASNGLYLYSGLUPHEILE
9   LEUSERSERLEULYSGLUSERILESERARG
10   ILELEULYSLEUTHRHISLEUASPLYSILE
11   PHELYSILETHRASPTHRVALGLUGLUALA

Samples:

sample_1: anti-sigma-factor antagonist 1.5 mM

sample_2: anti-sigma-factor antagonist, [U-15N], 2 mM

sample_3: anti-sigma-factor antagonist, [U-13C; U-15N], 1.2 mM

Ex-cond_1: pH: 7.0; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 5921
PDB
GB AAD36511 ACB09678 ADA67453 AGL50372 AHD18665
REF NP_229241 WP_004081714 YP_001739361 YP_003346867 YP_007977797
SP Q9X1F5
AlphaFold Q9X1F5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks