BMRB Entry 6228

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6228

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Title:
The Solution Structure of the FATC Domain of the Protein Kinase TOR Suggest a Redox Regulatory Mechanism
Deposition date:
2004-06-10
Original release date:
2005-03-17
Authors:
Dames, Sonja; Mulet, Jose; Rathgeb-Szabo, Klara; Hall, Michael; Grzesiek, Stephan
Citation:

Citation: Dames, S.; Mulet, J.; Rathgeb-Szabo, K.; Hall, M.; Grzesiek, S.. "The solution structure of the FATC domain of the protein kinase TOR suggests a role for redox-dependent structural and cellular stability"  J. Biol. Chem. 280, 20558-20564 (2005).
PubMed: 15772072

Assembly members:

Assembly members:
target of rapamycin, polymer, 33 residues, 3960.4 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
target of rapamycin: NELDVPEQVDKLIQQATSIE RLCQHYIGWCPFW

Data sets:
Data typeCount
13C chemical shifts154
15N chemical shifts37
1H chemical shifts211

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FATC domain of yeast TOR1, y1fatc1

Entities:

Entity 1, FATC domain of yeast TOR1, y1fatc 33 residues - 3960.4 Da.

1   ASNGLULEUASPVALPROGLUGLNVALASP
2   LYSLEUILEGLNGLNALATHRSERILEGLU
3   ARGLEUCYSGLNHISTYRILEGLYTRPCYS
4   PROPHETRP

Samples:

sample_1: target of rapamycin, [U-95% 13C; U-90% 15N], 0.5 mM; H2O 95%; D2O 5%; Tris 10 mM

sample_2: target of rapamycin, [U-95% 13C; U-90% 15N], 0.5 mM; Tris 10 mM

expcond1: pH: 6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D CBCANHnot availablenot availableexpcond1
3D CBCA(CO)NHnot availablenot availableexpcond1
3D HBHA(CBCACO)NHnot availablenot availableexpcond1
3D CCONH-TOCSYnot availablenot availableexpcond1
3D HCCH-TOCSYnot availablenot availableexpcond1
3D HNCOnot availablenot availableexpcond1
3D HAHB-COSYnot availablenot availableexpcond1
3D HNHAnot availablenot availableexpcond1
3D 15N ROESYnot availablenot availableexpcond1
3D 15N NOESYnot availablenot availableexpcond1
3D 13C NOESYnot availablenot availableexpcond1
13CO-{13Cg)-SED-15N HSQCnot availablenot availableexpcond1
15N-{13Cg)-SED-15N HSQCnot availablenot availableexpcond1
15N-1H-IPAP-HSQCnot availablenot availableexpcond1
T1 measurementnot availablenot availableexpcond1
T2 measurementnot availablenot availableexpcond1
{1H}-15N-NOEnot availablenot availableexpcond1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
EMBL CAA52849 CAA89594
GenBank AAB39292 AAB66881 EDN63385
PRF 2010264A
REF NP_012600
SWISS-PROT P35169

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks