BMRB Entry 6225
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6225
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Nguyen, Bao; Di Lello, Paola; Legault, Pascale; Ominchinski, James. "Letter to the Editor: 1H, 15N, and 13C Resonance Assignment of the Amino-terminal
Domain of Tfb1 subunit of yeast TFIIH" J. Biomol. NMR 31, 173-174 (2005).
Assembly members:
Yeast Tfb1, polymer, 119 residues, Formula weight is not available
Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Yeast Tfb1: PSHSGAAIFEKVSGIIAINE
DVSPAELTWRSTDGDKVHTV
VLSTIDKLQATPASSEKMML
RLIGKVDESKKRKDNEGNEV
VPKPQRHMFSFNNRTVMDNI
KMTLQQIISRYKDADGNSS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 807 |
| 13C chemical shifts | 497 |
| 15N chemical shifts | 124 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tfb1 | 1 |
Entities:
Entity 1, Tfb1 119 residues - Formula weight is not available
| 1 | PRO | SER | HIS | SER | GLY | ALA | ALA | ILE | PHE | GLU | ||||
| 2 | LYS | VAL | SER | GLY | ILE | ILE | ALA | ILE | ASN | GLU | ||||
| 3 | ASP | VAL | SER | PRO | ALA | GLU | LEU | THR | TRP | ARG | ||||
| 4 | SER | THR | ASP | GLY | ASP | LYS | VAL | HIS | THR | VAL | ||||
| 5 | VAL | LEU | SER | THR | ILE | ASP | LYS | LEU | GLN | ALA | ||||
| 6 | THR | PRO | ALA | SER | SER | GLU | LYS | MET | MET | LEU | ||||
| 7 | ARG | LEU | ILE | GLY | LYS | VAL | ASP | GLU | SER | LYS | ||||
| 8 | LYS | ARG | LYS | ASP | ASN | GLU | GLY | ASN | GLU | VAL | ||||
| 9 | VAL | PRO | LYS | PRO | GLN | ARG | HIS | MET | PHE | SER | ||||
| 10 | PHE | ASN | ASN | ARG | THR | VAL | MET | ASP | ASN | ILE | ||||
| 11 | LYS | MET | THR | LEU | GLN | GLN | ILE | ILE | SER | ARG | ||||
| 12 | TYR | LYS | ASP | ALA | ASP | GLY | ASN | SER | SER |
Samples:
sample_1: Yeast Tfb1 1.2 mM
sample_2: Yeast Tfb1, [U-98% 15N], 1.2 mM
sample_3: Yeast Tfb1, [U-98% 13C; U-98% 15N], 1.2 mM
Ex-cond_1: pH*: 6.5; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C CT-HSQC | not available | not available | not available |
| 1H-15N NOESY-HSQC | not available | not available | not available |
| HNCACB | not available | not available | not available |
| (HB)CBCA(CO)NNH | not available | not available | not available |
| HNCO | not available | not available | not available |
| H(CCO)NNH-TOCSY | not available | not available | not available |
| C(CO)NNH-TOCSY | not available | not available | not available |
| 1H-13C HMQC-NOESY | not available | not available | not available |
| 1H-15N NOESY_HSQC | not available | not available | not available |
| NOESY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
| (HB)CB(CGCD)HD | not available | not available | not available |
| (HB)CB(CGCDCE)HE | not available | not available | not available |
Software:
NMRPIPE - data processing
NMRVIEW v5.3 - data analysis
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
- Varian INOVA 500 MHz
Related Database Links:
| BMRB | 18229 18842 19791 |
| PDB | |
| DBJ | GAA22531 |
| EMBL | CAY78811 |
| GB | AAA35143 AAB64747 AAU09707 AHY75284 EDN60639 |
| REF | NP_010597 |
| SP | P32776 |
| TPG | DAA12150 |
| AlphaFold | P32776 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks