BMRB Entry 6216

Name: Solution structure of the third zinc finger domain of FOG-1
Published: 2005-02-08

Click here to enlarge.

PDB ID: 1srk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6216
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the third zinc finger domain of FOG-1

Deposition date:

2004-05-28

Original release date:

2005-02-08

Authors:

Simpson, R.; Lee, S.; Bartle, N.; Matthews, J.; Mackay, J.; Crossley, M.

Citation:

Citation: Simpson, R.; Lee, S.; Bartle, N.; Sum, E.; Visvader, J.; Matthews, J.; Mackay, J.; Crossley, M.. "A classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3" J. Biol. Chem. 279, 39789-39797 (2004).
PubMed: 15234987

Assembly members:

Assembly members:
ZFPM1, polymer, 35 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ZFPM1: GSSGKRPFVCRICLSAFTTK ANCARHLKVHTDTLS

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1
coupling_constants
- coupling_constants_set_1

Data type	Count
1H chemical shifts	220
coupling constants	31

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Zinc finger protein ZFPM1	1
2	ZINC (II) ION	2

Entities:

Entity 1, Zinc finger protein ZFPM1 35 residues - Formula weight is not available

1

GLY

SER

GLY

LYS

ARG

PRO

PHE

VAL

CYS

2

ARG

ILE

CYS

LEU

SER

ALA

PHE

THR

LYS

3

ALA

ASN

CYS

ALA

ARG

HIS

LEU

LYS

VAL

HIS

4

THR

ASP

THR

LEU

SER

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: ZFPM1 0.27 mM; ZnSO4 1 mM; TCEP 2 mM; H2O 95%; D2O 5%

sample_2: ZFPM1, [U-15N], 0.245 mM; ZnSO4 1 mM; TCEP 2 mM; H2O 95%; D2O 5%

sample_cond_1: ionic strength: 0 mM; pH: 5.6; pressure: 1 atm; temperature: 275 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	sample_cond_1
2D TOCSY	not available	not available	sample_cond_1
DQF-COSY	not available	not available	sample_cond_1
HNHA	not available	not available	sample_cond_1

Software:

xwinnmr v2.6 - processing

XEASY v1.3.13 - data analysis

DYANA v1.5 - refinement

ARIA v1.2 - structure solution

NMR spectrometers:

Bruker DRX 600 MHz