BMRB Entry 6211

Name: Chemical Shift Assignments for Carnobacteriocin B2 immunity protein
Published: 2004-11-12

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PDB ID: 1tdp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6211
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical Shift Assignments for Carnobacteriocin B2 immunity protein

Deposition date:

2004-05-23

Original release date:

2004-11-12

Authors:

Sprules, Tara; Kawulka, Karen; Vederas, John

Citation:

Citation: Sprules, Tara; Kawulka, Karen; Vederas, John. "NMR Solution Structure of ImB2, a Protein Conferring Immunity to Antimicrobial Activity of the Type IIa Bacteriocin, Carnobacteriocin B2 " Biochemistry 43, 11740-11749 (2004).
PubMed: 15362858

Assembly members:

Assembly members:
Carnobacteriocin B2 immunity protein, polymer, 111 residues, 12666 Da.

Natural source:

Natural source: Common Name: Carnobacterium maltaromaticum Taxonomy ID: 2751 Superkingdom: Eubacteria Kingdom: not available Genus/species: Carnobacterium piscicola

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Esherichia coli Vector: pMAL-c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Carnobacteriocin B2 immunity protein: MDIKSQTLYLNLSEAYKDPE VKANEFLSKLVVQCAGKLTA SNSENSYIEVISLLSRGISS YYLSHKRIIPSSMLTIYTQI QKDIKNGNIDTEKLRKYEIA KGLMSVPYIYF

Data sets:

assigned_chemical_shifts
- CbiB2_shifts
coupling_constants
- J_HNHA

Data type	Count
1H chemical shifts	748
13C chemical shifts	470
15N chemical shifts	112
coupling constants	71

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	imB2	1

Entities:

Entity 1, imB2 111 residues - 12666 Da.

1

MET

ASP

ILE

LYS

SER

GLN

THR

LEU

TYR

LEU

2

ASN

LEU

SER

GLU

ALA

TYR

LYS

ASP

PRO

GLU

3

VAL

LYS

ALA

ASN

GLU

PHE

LEU

SER

LYS

LEU

4

VAL

GLN

CYS

ALA

GLY

LYS

LEU

THR

ALA

5

SER

ASN

SER

GLU

ASN

SER

TYR

ILE

GLU

VAL

6

ILE

SER

LEU

SER

ARG

GLY

ILE

SER

7

TYR

LEU

SER

HIS

LYS

ARG

ILE

PRO

8

SER

MET

LEU

THR

ILE

TYR

THR

GLN

ILE

9

GLN

LYS

ASP

ILE

LYS

ASN

GLY

ASN

ILE

ASP

10

THR

GLU

LYS

LEU

ARG

LYS

TYR

GLU

ILE

ALA

11

LYS

GLY

LEU

MET

SER

VAL

PRO

TYR

ILE

TYR

12

PHE

Samples:

sample_1: Carnobacteriocin B2 immunity protein, [U-15N], 0.8 mM; DSS 0.025 mM; sodium phosphate 20 mM; sodium chloride 25 mM

sample_2: Carnobacteriocin B2 immunity protein, [U-13C; U-15N], 0.8 mM; DSS 0.025 mM; sodium phosphate 20 mM; sodium chloride 25 mM

condition_1: pH: 6.6; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N-HSQC NOESY	not available	not available	condition_1
15N-HSQC TOCSY	not available	not available	condition_1
13C-HSQC NOESY	not available	not available	condition_1
13C-HSQC TOCSY	not available	not available	condition_1
HNHA	not available	not available	condition_1
13C-HSQC	not available	not available	condition_1
HNCACB	not available	not available	condition_1
HNCA	not available	not available	condition_1
HNCO	not available	not available	condition_1
CBCACONH	not available	not available	condition_1
15N-HSQC	not available	not available	condition_1

Software:

NMRPipe v2.2 - data processing

NMRView v5.0 - data analysis

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 800 MHz

PDB	1TDP
GB	AAA72432 AAB18990 AAB81311
SP	P38582
AlphaFold	P38582