BMRB Entry 6209
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6209
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Song, Jikui; Zhao, Qin; Thao, Sandy; Frederick, Ronnie; Markley, John. "Letter to the Editor: Solution Structure of a Calmodulin-Like Calcium-Binding
Domain from Arabidopsis thaliana" J. Biomol. NMR 30, 451-456 (2004).
Assembly members:
N-terminal domain of At3g03410.1, polymer, 67 residues, Formula weight is not available
Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDEST17
Entity Sequences (FASTA):
N-terminal domain of At3g03410.1: SSAKRVFEKFDKNKDGKLSL
DEFREVALAFSPYFTQEDIV
KFFEEIDVDGNGELNADEFT
SCIEKML
- assigned_chemical_shifts
- RDCs
| Data type | Count |
| 1H chemical shifts | 395 |
| 13C chemical shifts | 261 |
| 15N chemical shifts | 69 |
| residual dipolar couplings | 55 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Calmodulin like protein | 1 |
Entities:
Entity 1, Calmodulin like protein 67 residues - Formula weight is not available
| 1 | SER | SER | ALA | LYS | ARG | VAL | PHE | GLU | LYS | PHE | ||||
| 2 | ASP | LYS | ASN | LYS | ASP | GLY | LYS | LEU | SER | LEU | ||||
| 3 | ASP | GLU | PHE | ARG | GLU | VAL | ALA | LEU | ALA | PHE | ||||
| 4 | SER | PRO | TYR | PHE | THR | GLN | GLU | ASP | ILE | VAL | ||||
| 5 | LYS | PHE | PHE | GLU | GLU | ILE | ASP | VAL | ASP | GLY | ||||
| 6 | ASN | GLY | GLU | LEU | ASN | ALA | ASP | GLU | PHE | THR | ||||
| 7 | SER | CYS | ILE | GLU | LYS | MET | LEU |
Samples:
sample_1: N-terminal domain of At3g03410.1, [U-15N], 1 mM; DTT 5 mM; Bis-Tris 20 mM; CaCl2 5 mM; DSS 1 mM
2: N-terminal domain of At3g03410.1, [U-15N; U-13C], 1 mM; DTT 5 mM; Bis-Tris 20 mM; CaCl2 5 mM; DSS 1 mM
3: N-terminal domain of At3g03410.1, [U-15N; U-13C], 1 mM; DTT 5 mM; Bis-Tris 20 mM; CaCl2 5 mM; DSS 1 mM; PEG 5%
Conditions_1: pH: 6.4; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCACB | not available | not available | Conditions_1 |
| HN(CO)CACB | not available | not available | Conditions_1 |
| N15 edited NOESY | not available | not available | Conditions_1 |
| N15 edited TOCSY | not available | not available | Conditions_1 |
| IPAP-HSQC | not available | not available | Conditions_1 |
Software:
XWINNMR v3.2, Bruker - Data collection
NMR spectrometers:
- Bruker DMX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks